Marine Compound Exerts Antiaging Effect in Human Endothelial Progenitor Cells via Increasing Sirtuin1 Expression

Area of Science:

• Cardiovascular Disease Research
• Cellular Aging Mechanisms
• Marine Natural Products

Background:

• Aging increases cardiovascular disease risk.
• Endothelial progenitor cells (hEPCs) are crucial for vascular health.
• Marine-derived compounds are explored for therapeutic potential.

Purpose of the Study:

• To investigate the effects of compound 3 (C3) on senescence in human endothelial progenitor cells (hEPCs).
• To elucidate the molecular mechanisms underlying C3's protective effects against age-related endothelial dysfunction.

Main Methods:

• Induction of hEPC senescence using angiotensin II (AngII).
• Assessment of senescence markers (SA-β-galactosidase, telomerase activity, viability).
• Analysis of key signaling pathways (SIRT1, AMPK, Akt) and intervention with specific inhibitors.

Main Results:

• AngII induced hEPC senescence, decreasing telomerase activity and viability.
• C3 dose-dependently reversed AngII-induced senescence and restored cell function.
• C3 increased SIRT1 expression and modulated AMPK/Akt phosphorylation, effects dependent on SIRT1 activity.

Conclusions:

• Compound 3 protects hEPCs from AngII-induced senescence via SIRT1 upregulation and AMPK/Akt pathway regulation.
• C3 demonstrates potential as a therapeutic agent for aging-related cardiovascular disorders.
• Targeting hEPC senescence with C3 may mitigate endothelial dysfunction in the elderly.