Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Emerging therapeutic strategies for mitochondrial DNA-related diseases.

Cell reports. Medicine·2026
Same author

A SET domain-containing protein and HCF-1 maintain transgenerational epigenetic memory.

Nature communications·2026
Same author

Expanding research and care for Leigh syndrome: efforts of a patient-led advocacy organization.

Research involvement and engagement·2025
Same author

An inherited mitochondrial DNA mutation remodels inflammatory cytokine responses in macrophages and in vivo in mice.

Nature communications·2025
Same author

Genome-wide rules of transcription factor cooperativity revealed through <i>in silico</i> binding site ablation.

bioRxiv : the preprint server for biology·2025
Same author

Clu1/Clu form mitochondria-associated granules upon metabolic transitions and regulate mitochondrial protein translation via ribosome interactions.

PLoS genetics·2025

Related Experiment Video

Updated: Jul 11, 2025

Author Spotlight: Two-Step Tag-Free Isolation of Mitochondria for Improved Protein Discovery and Quantification
09:04

Author Spotlight: Two-Step Tag-Free Isolation of Mitochondria for Improved Protein Discovery and Quantification

Published on: June 2, 2023

6.0K

Protocol to study human mitochondrial ribosome using quantitative density gradient analysis by mass spectrometry and

Petra Páleníková1, Michal Minczuk1, Pedro Rebelo-Guiomar1

  • 1MRC Mitochondrial Biology Unit, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK.

STAR Protocols
|November 17, 2023
PubMed
Summary

This study introduces a new method for analyzing large protein complexes, like the human mitochondrial ribosome, under near-native conditions. This approach combines density gradient ultracentrifugation with mass spectrometry for detailed molecular complex analysis.

Keywords:
BioinformaticsProtein Expression and PurificationProteomics

More Related Videos

Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA
12:35

Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA

Published on: November 14, 2017

9.5K
Author Spotlight: Mitochondrial Remodeling in Skeletal Muscle
10:53

Author Spotlight: Mitochondrial Remodeling in Skeletal Muscle

Published on: December 1, 2023

3.4K

Related Experiment Videos

Last Updated: Jul 11, 2025

Author Spotlight: Two-Step Tag-Free Isolation of Mitochondria for Improved Protein Discovery and Quantification
09:04

Author Spotlight: Two-Step Tag-Free Isolation of Mitochondria for Improved Protein Discovery and Quantification

Published on: June 2, 2023

6.0K
Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA
12:35

Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA

Published on: November 14, 2017

9.5K
Author Spotlight: Mitochondrial Remodeling in Skeletal Muscle
10:53

Author Spotlight: Mitochondrial Remodeling in Skeletal Muscle

Published on: December 1, 2023

3.4K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Proteomics

Background:

  • Studying large, dynamic macromolecular complexes is challenging with traditional methods like immunoblotting.
  • Conventional techniques often disrupt the native structure of these complexes.

Purpose of the Study:

  • To present a flexible protocol for analyzing macromolecular complexes in near-native conditions.
  • To enable standardized analysis of large, multi-component cellular machinery.

Main Methods:

  • Development of a protocol for near-native analysis of macromolecular complexes.
  • Application of density gradient ultracentrifugation coupled with quantitative mass spectrometry.
  • Utilizing a computational analysis pipeline (ComPrAn) for data interpretation.

Main Results:

  • Successful standardized analysis of the human mitochondrial ribosome, a complex of 82 proteins.
  • Demonstration of a flexible setup applicable to various cellular targets.
  • Validation of the combined ultracentrifugation and mass spectrometry approach.

Conclusions:

  • The presented protocol offers a robust method for studying complex macromolecular assemblies.
  • This technique facilitates deeper understanding of cellular machinery in a near-native state.
  • The protocol is adaptable for diverse biological research questions involving large complexes.