Related Experiment Video
Updated: Aug 7, 2026
Detecting, Visualizing and Quantitating the Generation of Reactive Oxygen Species in an Amoeba Model System
Published on: November 5, 2013
A fluorescence "turn-on" sensor for ascorbic acid in fruit juice and beverage based on ascorbate oxidase-like
Zhen Lin1, Qi Zeng2, Wensong Yao3
1Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350122, China; Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, Fujian Medical University, Fuzhou 350122, China.
Abstract:
Citric acid-derived carbon dots (CA-CDs) without any modifications were found to have the ascorbate oxidase (AAO)-like activity. The CA-CDs have high affinity for ascorbic acid (AA), which is similar to natural AAO. The robustness of CA-CDs is greater than that of AAO. Based on the AAO mimetic activity of CA-CDs, a sensitive turn-on mode and natural enzyme-free fluorescence detection method has been developed for AA in some fruit juice and beverage samples with satisfied recoveries. This study provides CDs-based AAO mimetic nanozymes to replace the expensive natural enzymes or heavy metal-based nanozymes, which will show great potential in biological and food assays.
More Related Videos
11:56A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
Published on: April 11, 2014
04:53Detection of Total Reactive Oxygen Species in Adherent Cells by 2',7'-Dichlorodihydrofluorescein Diacetate Staining
Published on: June 23, 2020
