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Updated: Jul 11, 2025

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli
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Protein engineering using mutability landscapes: Controlling site-selectivity of P450-catalyzed steroid hydroxylation

Huili Yu1, Xiaodong Zhang1, Carlos G Acevedo-Rocha2

  • 1State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Key Laboratory of Industrial Biotechnology, School of life science, Hubei University, Wuhan, P.R. China.

Methods in Enzymology
|November 17, 2023
PubMed

Keywords:
Cytochrome P450Directed evolutionMutability landscapesProtein engineeringRegio-/diastereoselective steroid hydroxylation

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