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Updated: Jul 11, 2025

A General Method for Detecting Nitrosamide Formation in the In Vitro Metabolism of Nitrosamines by Cytochrome P450s
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A General Method for Detecting Nitrosamide Formation in the In Vitro Metabolism of Nitrosamines by Cytochrome P450s

Published on: September 25, 2017

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Direct aromatic nitration by bacterial P450 enzymes.

Manyun Chen1, Vanisa Petriti1, Amit Mondal1

  • 1Department of Medicinal Chemistry, Center for Natural Products, Drug Discovery and Development, College of Pharmacy, University of Florida, Gainesville, FL, United States.

Methods in Enzymology
|November 17, 2023
PubMed
Summary

Researchers engineered a bacterial cytochrome P450 enzyme, TxtE, into a self-sufficient biocatalyst for aromatic nitration. This novel biocatalyst, TxtE fused with a reductase domain, shows broad substrate scope for synthesizing nitroaromatics efficiently and sustainably.

Keywords:
Chemical nitrationCoupling efficiencyLinker lengthNitro aromaticsP450 TxtEReductase domainRegioselectivitySelf-sufficientSpectral analysisTotal turnover number

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