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A Technical Guide for Performing Spectroscopic Measurements on Metal-Organic Frameworks
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Visible light-driven oxidation of non-native substrate by laccase attached on Ru-based metal-organic frameworks
Wenguang Huang1, Wentao Zhang2, Guantongyi Chen1
1State Environmental Protection Key Laboratory of Environmental Pollution Health Risk Assessment, South China Institute of Environmental Sciences, Ministry of Ecology and Environment of PRC, Guangzhou 510655, China.
Abstract:
Light-induced electron transfer can broaden the substrate range of metalloenzyme. However, the efficiency of photo-enzyme coupling is limited by the poor combination of photosensitizer or photocatalyst with enzyme. Herein, we prepared the nano-photocatalyst MIL-125-NH2@Ru(bpy) by in site embedding ruthenium pyridine-diimine complex [Ru(bpy)3]2+ into metal organic frameworks MIL-125-NH2 and associated it with multicopper oxidase (MCO) laccase. Compared to [Ru(bpy)3]2+, the coupling efficiency of MIL-125-NH2@Ru(bpy)3 for enzymatic oxygen reduction increased by 35.7%. A series of characterizations confirmed that the amino group of laccase formed chemical bonds with the surface defects or hydrophobic groups of MIL-125-NH2@Ru(bpy)3. Consequently, the tight binding accelerated the quenching process and electron transfer between laccase and the immobilized ruthenium pyridine-diimine complex. This work would open an avenue for the synthesis of MOFs photocatalyst towards photo-enzyme coupling.
