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Mast Cell Concentrations in Pancreatic Disease Processes.
Lindsey Wynn1, Victoria Hodges2, Sheila Criswell1
1Department of Diagnostic and Health Sciences, University of Tennessee Health Science Center.
Applied Immunohistochemistry & Molecular Morphology : AIMM
|November 20, 2023
Summary
Mast cell counts in pancreatic tissues were higher with immunohistochemistry markers (tryptase, CD117) than Wright stain. Fibrotic conditions like pancreatitis and adenocarcinoma showed increased mast cell concentrations.
Area of Science:
- Gastroenterology
- Oncology
- Immunology
Background:
- Mast cells play a role in various pathologic processes.
- Accurate enumeration of mast cells is crucial for understanding their influence.
- Histologic staining techniques vary in their efficacy for mast cell detection.
Purpose of the Study:
- To compare mast cell enumeration using Wright stain versus immunohistochemistry markers (tryptase, CD117) in human pancreatic tissues.
- To investigate mast cell concentrations in normal pancreas, benign fibrosis, endocrine tumors, and adenocarcinoma.
- To explore the relationship between pancreatic fibrosis and mast cell infiltration.
Main Methods:
- Evaluation of 77 human pancreatic tissue samples.
- Application of Wright stain for mast cell identification.
- Immunohistochemistry using markers for tryptase and CD117.
- Quantitative analysis of mast cell counts across different tissue types.
Main Results:
- Mast cell counts were significantly higher using tryptase and CD117 compared to Wright stain.
- Endocrine tumors and normal pancreatic tissues exhibited lower mast cell counts.
- Benign fibrosis and adenocarcinoma showed significantly increased mast cell concentrations.
Conclusions:
- Immunohistochemistry (tryptase, CD117) is more sensitive for mast cell enumeration in pancreatic tissues than Wright stain.
- Increased mast cell concentrations are associated with fibrotic pancreatic conditions, including pancreatitis and adenocarcinoma.
- Mast cells may contribute to the fibrotic nature of pancreatic adenocarcinoma.
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