Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mitochondrial Membranes01:45

Mitochondrial Membranes

11.1K
A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
11.1K
Translocation of Proteins into the Mitochondria01:19

Translocation of Proteins into the Mitochondria

3.1K
Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
3.1K
Mitochondria01:37

Mitochondria

13.4K
Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
13.4K
Mitochondrial Protein Sorting01:39

Mitochondrial Protein Sorting

4.3K
Mitochondria are double-membrane organelles of the eukaryotes involved in cellular metabolism, signaling, ATP synthesis, and programmed cell death.  Each of these processes requires specific proteins and enzymes that must be correctly sorted to the right mitochondrial subcompartment for the proper functioning of the organelle.
Most of these mitochondrial proteins are encoded by the nucleus and imported to the mitochondria as unfolded or loosely folded precursors. Mitochondrial precursors...
4.3K
Animal Mitochondrial Genetics02:59

Animal Mitochondrial Genetics

7.6K
Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...
7.6K
Mitochondrial Precursor Proteins01:39

Mitochondrial Precursor Proteins

2.6K
Mitochondrial precursors are partially unfolded or loosely folded polypeptide chains. Newly synthesized precursors are inhibited from spontaneously folding into their native conformation by the cytosolic chaperones, heat shock proteins 70 (Hsp70), and mitochondrial import stimulation factors (MSFs). Precursors bound to MSFs are guided to the TOM70-TOM37 receptors, while precursors bound to Hsp70  chaperones are targetted to TOM20-TOM22 receptor complexes.
Most of the mitochondrial...
2.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparative genomic analysis of clinically relevant human skin-associated fungi.

Nature communications·2026
Same author

Investigating the relationship between ATP synthase and the TCA cycle by crosslinking mass spectrometry.

Nature communications·2026
Same author

Proteomics reveals spatial and molecular heterogeneities in advanced atherosclerotic carotid artery plaques.

Nature cardiovascular research·2026
Same author

Author Correction: Regulatory T cells in the mouse hypothalamus control immune activation and ameliorate metabolic impairments in high-calorie environments.

Nature communications·2026
Same author

Cysteine availability tunes ubiquitin signaling via inverse stability of LRRC58 E3 ligase and its substrate CDO1.

Nature communications·2026
Same author

Deeper is not always better in plasma proteomics.

Nature biotechnology·2026
Same journal

TERT drives liver tumorigenesis beyond telomere elongation.

Life science alliance·2026
Same journal

Deletion of exon 2 in ALS-linked <i>Sptlc1</i> causes lethality in homozygous mice but not in heterozygotes.

Life science alliance·2026
Same journal

Peroxisomal interactome mapping enables network-based modelling of function and disease.

Life science alliance·2026
Same journal

UCA1 lncRNA regulates γ-globin expression by modulating the miR-148b/BCL11A axis.

Life science alliance·2026
Same journal

Democratized single-cell proteomics resolves cell state heterogeneity in skin tumors.

Life science alliance·2026
Same journal

Cross-tissue dual-omics analysis reveals molecular programs linked to myopia susceptibility and progression.

Life science alliance·2026
See all related articles

Related Experiment Video

Updated: Jul 10, 2025

Author Spotlight: Uncovering the Role of Mitochondrial Calcium Phosphate in Heart Failure and Bioenergetics
07:03

Author Spotlight: Uncovering the Role of Mitochondrial Calcium Phosphate in Heart Failure and Bioenergetics

Published on: August 23, 2024

814

Mitochondrial phosphoproteomes are functionally specialized across tissues.

Fynn M Hansen1, Laura S Kremer2, Ozge Karayel1

  • 1Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Martinsried, Germany.

Life Science Alliance
|November 20, 2023
PubMed
Summary
This summary is machine-generated.

This study maps mitochondrial proteins and their phosphorylation across mouse tissues, revealing tissue-specific regulation crucial for organelle function and health. The findings highlight phosphorylation

More Related Videos

Author Spotlight: Two-Step Tag-Free Isolation of Mitochondria for Improved Protein Discovery and Quantification
09:04

Author Spotlight: Two-Step Tag-Free Isolation of Mitochondria for Improved Protein Discovery and Quantification

Published on: June 2, 2023

6.0K
Author Spotlight: Unveiling Oxidative Phosphorylation System Dynamics and Mitochondrial Roles in Health and Disease
05:45

Author Spotlight: Unveiling Oxidative Phosphorylation System Dynamics and Mitochondrial Roles in Health and Disease

Published on: May 3, 2024

1.2K

Related Experiment Videos

Last Updated: Jul 10, 2025

Author Spotlight: Uncovering the Role of Mitochondrial Calcium Phosphate in Heart Failure and Bioenergetics
07:03

Author Spotlight: Uncovering the Role of Mitochondrial Calcium Phosphate in Heart Failure and Bioenergetics

Published on: August 23, 2024

814
Author Spotlight: Two-Step Tag-Free Isolation of Mitochondria for Improved Protein Discovery and Quantification
09:04

Author Spotlight: Two-Step Tag-Free Isolation of Mitochondria for Improved Protein Discovery and Quantification

Published on: June 2, 2023

6.0K
Author Spotlight: Unveiling Oxidative Phosphorylation System Dynamics and Mitochondrial Roles in Health and Disease
05:45

Author Spotlight: Unveiling Oxidative Phosphorylation System Dynamics and Mitochondrial Roles in Health and Disease

Published on: May 3, 2024

1.2K

Area of Science:

  • Cell Biology
  • Biochemistry
  • Proteomics

Background:

  • Mitochondrial dysfunction contributes to tissue-specific human diseases.
  • Mitochondrial proteomes are specialized for diverse tissue requirements.
  • Phosphorylation is increasingly recognized as a key regulator of mitochondrial function.

Purpose of the Study:

  • To quantitatively profile mitochondrial proteomes and phosphoproteomes across various mouse tissues.
  • To create a comprehensive resource of core and tissue-specific mitochondrial proteins.
  • To investigate tissue-specific phosphorylation patterns and their regulatory roles.

Main Methods:

  • Isolation of mitochondria from mouse heart, skeletal muscle, brown adipose tissue, kidney, liver, brain, and spleen.
  • High-resolution mass spectrometry (MS)-based quantitative proteomics and phosphoproteomics.
  • Bioinformatic analysis to predict kinase-substrate associations and identify phosphorylation clusters.

Main Results:

  • Comprehensive quantitative map of mitochondrial proteins and phosphoproteins across seven mouse tissues.
  • Identification of core and tissue-specific mitochondrial proteins, available at mitophos.de.
  • Evidence for tissue-specific regulation at the phosphoproteome level, with examples in dynamin-related protein 1 and MIGA2.

Conclusions:

  • Mitochondrial phosphorylation patterns are tissue-specific and play a role in regulating mitochondrial dynamics and function.
  • The generated resource provides valuable insights into tissue-specific mitochondrial biology and pathophysiology.
  • This work advances our understanding of how phosphorylation fine-tunes mitochondrial activities in different cellular contexts.