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Agroinoculation of Geminiviral Infectious Clones into Plants
Ivair J Morais1, Yanca F M Ferreira2, Erich Y T Nakasu2
1Department of Phytopathology, University of Brasília, Brasília, DF, Brazil.
Abstract:
Most geminiviruses are not transmitted by mechanical inoculation. Therefore, pathogenicity and plant-pathogen interaction studies rely on agroinoculation using infectious clones, which involves cloning the geminiviral genome in a binary vector (see previous chapter for details). A suspension containing the infectious clone inserted into Agrobacterium tumefaciens cells is then inoculated into plants, i.e., agroinoculated. Below is a simple protocol for agroinoculation of an infectious geminivirus clone into plants.
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