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Updated: Jul 3, 2026

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Preparation and Using Phantom Lesions to Practice Fine Needle Aspiration Biopsies
Published on: September 29, 2009
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Benchtop fine needle aspirations: An untapped source of cytologic educational material
Yubo Wu1,2, Marcos Lepe1,2
1Department of Pathology, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA.
Diagnostic Cytopathology
|November 22, 2023
Summary
Benchtop fine needle aspirations (FNAs) using fresh surgical specimens provide a practical training method for cytopathology. This technique enhances skills in specimen adequacy and slide preparation, offering valuable cyto-histo correlation.
Area of Science:
- Cytopathology
- Surgical Pathology
- Medical Education
Background:
- Fine needle aspirations (FNAs) are crucial for diagnosing various lesions.
- Training in FNA performance, specimen adequacy, and slide smearing presents challenges.
- Limited FNA volume in some institutions restricts hands-on practice.
Purpose of the Study:
- To evaluate the feasibility and practicality of benchtop FNAs of fresh surgical specimens as a training tool.
- To demonstrate the utility of this method for improving cytopathology skills.
- To address the need for simulated practice scenarios in FNA training.
Main Methods:
- Performed benchtop FNAs on various fresh surgical specimens, primarily nephrectomies.
- Documented the technique and generated cytology slides.
- Correlated generated slides with surgical pathology findings.
Main Results:
- Benchtop FNAs generated high-quality cytology slides.
- The method facilitated immediate gross-microscopic and cyto-histo correlation.
- Demonstrated the potential for artifact introduction and the need for immediate specimen access.
Conclusions:
- Benchtop FNAs of fresh surgical specimens are a valuable addition to cytopathology training.
- This technique offers advantages over traditional methods, especially for low-volume FNA institutions.
- Enhances training by providing realistic practice and direct correlation opportunities.

