Mass Spectrometry-Based Multiomics Identifies Metabolic Signatures of Sarcopenia in Rhesus Monkey Skeletal Muscle.

Area of Science:

• Gerontology
• Muscle Physiology
• Metabolomics

Background:

• Sarcopenia, characterized by age-related loss of muscle mass and function, has unclear underlying molecular mechanisms.
• Understanding these mechanisms is crucial for developing effective interventions.
• Omics technologies provide powerful tools for dissecting complex biological processes like muscle aging.

Purpose of the Study:

• To identify molecular signatures of sarcopenia using mass spectrometry-based proteomic and metabolomic analyses.
• To investigate age-related changes in skeletal muscle of rhesus monkeys.
• To uncover new insights into the molecular basis of sarcopenia.

Main Methods:

• Mass spectrometry-based proteomic and untargeted metabolomic analyses were performed on vastus lateralis muscle samples.
• Samples were obtained from young, middle-aged, and older rhesus monkeys.
• Pathway analysis was used to interpret age-responsive molecular changes.

Main Results:

• Proteomic analysis identified age-related changes in proteins involved in adenosine triphosphate/adenosine monophosphate metabolism and fatty acid beta-oxidation.
• Metabolomic analysis revealed age-dependent alterations in metabolites, primarily related to energy metabolism, including fatty acid beta-oxidation.
• Pathway analysis indicated significant changes in muscle structure, contraction, and lipid, carbohydrate, and purine metabolism.

Conclusions:

• This study identifies novel metabolic signatures associated with sarcopenia in rhesus monkeys.
• The findings provide new insights into the molecular mechanisms of age-related muscle decline.
• These discoveries can aid in the evaluation and monitoring of sarcopenia therapeutics.