Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Electron Microscopy01:25

Overview of Electron Microscopy

9.2K
The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
9.2K
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

4.2K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
4.2K
Electronic Distance Measuring Instruments01:30

Electronic Distance Measuring Instruments

37
Electronic Distance Measuring Instruments (EDMs) are essential tools in modern surveying, offering precise distance measurements by emitting electromagnetic signals and calculating the time required for these signals to travel to a target and return. Two primary types of signals are used in EDMs — light waves and microwaves — each suited to specific environmental and distance requirements. Light-wave-based EDMs utilize either infrared or laser light, providing high accuracy over short...
37
Gas Chromatography: Types of Detectors-II01:19

Gas Chromatography: Types of Detectors-II

391
In gas chromatography, different detectors are employed to meet specific analytical needs. These detectors are often categorized based on their detection mechanisms and the types of compounds they are best suited to analyze. Thermal Conductivity Detectors (TCD), Flame Ionization Detectors (FID), and Electron Capture Detectors (ECD) represent common categories, each with unique operating principles and applications. However, beyond these, several other detectors are designed for more specialized...
391
Transmission Electron Microscopy01:15

Transmission Electron Microscopy

5.5K
In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400...
5.5K
Atomic Emission Spectroscopy: Instrumentation01:22

Atomic Emission Spectroscopy: Instrumentation

497
The instrumentation of atomic emission spectrometry (AES) involves various components, including atomization devices that convert samples into gas-phase atoms and ions. There are two main types of atomization devices: continuous and discrete atomizers.  Continuous atomizers, like plasmas and flames, introduce samples in a constant stream, while discrete atomizers inject individual samples using syringes or autosamplers. The most common discrete atomizer is the electrothermal atomizer.
497

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Membrane protein structure and dynamics probed by MicroED.

Biochemical Society transactions·2026
Same author

pH-mediated activation of the lysosomal arginine sensor SLC38A9.

FEBS letters·2026
Same author

Direct from the seed: an atomic resolution protein structure by ab initio MicroED.

Nature communications·2026
Same author

Rapid Structural Analysis of Natural Products Using MicroED.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

β-barrels from short macrocyclic peptides.

Chemical communications (Cambridge, England)·2026
Same author

Conformational Trajectory of the Molecular Chameleon Grazoprevir From Formulation to Target-Bound.

Chemistry (Weinheim an der Bergstrasse, Germany)·2025
Same journal

Structure of Perinereis linea erythrocruorin reveals a compact extracellular globin megacomplex.

Structure (London, England : 1993)·2026
Same journal

Meet the author: Stephen Brohawn.

Structure (London, England : 1993)·2026
Same journal

Tetraspanins bring Norrin into focus: Structural insights into ligand-specific Wnt signaling.

Structure (London, England : 1993)·2026
Same journal

Uncovering subtype-selective activation of the K<sub>Ca</sub>3.1 channel by SKA-111.

Structure (London, England : 1993)·2026
Same journal

Identification and structure determination of a type III-Bv CRISPR complex that post-translationally modifies an associated toxin.

Structure (London, England : 1993)·2026
Same journal

Cryo-EM structure of the Arabidopsisthaliana ribosome in translating and non-translating states.

Structure (London, England : 1993)·2026
See all related articles

Related Experiment Video

Updated: Jul 10, 2025

Measurement of Total Calcium in Neurons by Electron Probe X-ray Microanalysis
11:42

Measurement of Total Calcium in Neurons by Electron Probe X-ray Microanalysis

Published on: November 20, 2013

12.1K

Electron counting with direct electron detectors in MicroED.

Johan Hattne1, Max T B Clabbers1, Michael W Martynowycz1

  • 1Howard Hughes Medical Institute, University of California, Los Angeles, Los Angeles, CA 90095, USA; Department of Biological Chemistry, University of California, Los Angeles, Los Angeles, CA 90095, USA.

Structure (London, England : 1993)
|November 22, 2023
PubMed
Summary
This summary is machine-generated.

Electron-counting detectors enhance cryogenic electron microscopy (cryo-EM) data collection speed and accuracy. Minimizing coincidence loss in these detectors offers significant advantages for MicroED applications.

Keywords:
MicroEDcryo-EMelectron countingmicrocrystal electron diffraction

More Related Videos

Quantitative Atomic-Site Analysis of Functional Dopants/Point Defects in Crystalline Materials by Electron-Channeling-Enhanced Microanalysis
07:24

Quantitative Atomic-Site Analysis of Functional Dopants/Point Defects in Crystalline Materials by Electron-Channeling-Enhanced Microanalysis

Published on: May 10, 2021

6.0K
Microcrystal Electron Diffraction of Small Molecules
09:48

Microcrystal Electron Diffraction of Small Molecules

Published on: March 15, 2021

6.7K

Related Experiment Videos

Last Updated: Jul 10, 2025

Measurement of Total Calcium in Neurons by Electron Probe X-ray Microanalysis
11:42

Measurement of Total Calcium in Neurons by Electron Probe X-ray Microanalysis

Published on: November 20, 2013

12.1K
Quantitative Atomic-Site Analysis of Functional Dopants/Point Defects in Crystalline Materials by Electron-Channeling-Enhanced Microanalysis
07:24

Quantitative Atomic-Site Analysis of Functional Dopants/Point Defects in Crystalline Materials by Electron-Channeling-Enhanced Microanalysis

Published on: May 10, 2021

6.0K
Microcrystal Electron Diffraction of Small Molecules
09:48

Microcrystal Electron Diffraction of Small Molecules

Published on: March 15, 2021

6.7K

Area of Science:

  • Structural Biology
  • Microscopy

Background:

  • Electron-counting detectors offer high sensitivity and rapid readout for cryo-electron microscopy (cryo-EM).
  • These detectors are particularly beneficial for Microcrystal Electron Diffraction (MicroED) of macromolecular crystals, where signals are weak.
  • Reduced electron fluence minimizes radiation damage, preserving data integrity.

Purpose of the Study:

  • To evaluate the performance of electron-counting detectors in cryo-EM and MicroED.
  • To address challenges associated with electron-counting detectors, specifically coincidence loss at low resolutions.

Main Methods:

  • Utilizing electron-counting detectors for data acquisition in cryo-EM.
  • Applying Microcrystal Electron Diffraction (MicroED) techniques.
  • Analyzing data quality considering detector linearity and coincidence loss.

Main Results:

  • Electron-counting detectors enable faster and more accurate cryo-EM data collection.
  • High sensitivity and rapid readout improve MicroED of macromolecular crystals.
  • Concerns regarding radiation damage are mitigated by lower electron fluence.

Conclusions:

  • Electron-counting detectors show high potential for advancing cryo-EM and MicroED.
  • Minimizing coincidence loss is crucial for optimal data quality with these detectors.
  • Widespread adoption in cryo-EM facilities is anticipated with careful data collection strategies.