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Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Published on: February 6, 2020

Ionic liquid-caged nucleic acids enable active folding-based molecular recognition with hydrolysis resistance.

Byunghwa Kang¹, Soyeon V Park¹, Seung Soo Oh^1,2

¹Department of Materials Science and Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Gyeongbuk 37673, South Korea.

Nucleic Acids Research

|November 23, 2023

Summary

Choline dihydrogen phosphate protects nucleic acids from degradation by preventing hydrolysis. This ionic liquid preserves nucleic acid functions, even in the presence of nucleases, for diverse applications.

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Area of Science:

Biochemistry
Materials Science
Molecular Biology

Background:

Nucleic acids possess diverse functions beyond genetic storage, including catalysis and recognition.
Nucleic acids are susceptible to hydrolysis, limiting their stability and applications.

Purpose of the Study:

To investigate the protective effect of choline dihydrogen phosphate on nucleic acids.
To demonstrate the preservation of nucleic acid functions in this ionic liquid.

Main Methods:

Utilized choline dihydrogen phosphate as a protective medium for nucleic acids.
Assessed nucleic acid stability and functional activity in the presence of nucleases.

Main Results:

Choline dihydrogen phosphate prevents phosphodiester hydrolysis of nucleic acids via a cage effect.
Nucleic acid half-lives were extended up to 6,500,000 times.
Key nucleic acid functions, including mRNA detection, aptamer recognition, and ribozyme activity, were maintained with high fidelity.
Molecular functions remained intact even in complex environments like nuclease cocktails and human saliva.

Conclusions:

Choline dihydrogen phosphate offers a universal solution for stabilizing nucleic acids.
This ionic liquid enables robust nucleic acid applications by preventing degradation and preserving function.