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3D Printed Tubulointerstitium Chip as an In Vitro Testing Platform.

Gabriele Addario1, Daphne Eussen1, Sonja Djudjaj2

  • 1Maastricht University, MERLN Institute for Technology-Inspired Regenerative Medicine, Complex Tissue Regeneration Department, Maastricht, 6229 ER, The Netherlands.

Macromolecular Bioscience
|November 24, 2023
PubMed
Summary

Researchers developed a 3D-printed kidney-on-a-chip model to better test drugs for chronic kidney disease (CKD). This organ-on-a-chip platform mimics kidney tubules and blood vessels for improved in vitro drug screening.

Keywords:
3D printingchip platformin vitro modelkidneytubulointerstitium

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Area of Science:

  • Biomedical Engineering
  • Regenerative Medicine
  • Organ-on-a-Chip Technology

Background:

  • Chronic kidney disease (CKD) is a significant global health issue with limited therapeutic options.
  • Current in vitro models often lack the physiological relevance and reproducibility needed for effective drug testing.
  • Nephron-based models are crucial for understanding kidney function and disease progression.

Purpose of the Study:

  • To develop a novel 3D-printed organ-on-a-chip platform that replicates kidney tubule and capillary structures.
  • To create a physiologically relevant in vitro model for studying the renal tubulointerstitium.
  • To establish a cost-effective, reproducible, and automated manufacturing process for kidney-on-a-chip devices.

Main Methods:

  • Utilized extrusion-based 3D printing with sacrificial fibers to create tubular platforms.
  • Seeded the chip with primary murine tubular epithelial cells and human umbilical vein endothelial cells.
  • Investigated channel geometry, reproducibility, cell adhesion coatings, and cell marker expression.

Main Results:

  • Successfully manufactured perfusable circular cross-section channels mimicking renal tubules and capillary networks.
  • Integrated an extracellular matrix gel analogue to represent the renal tubulointerstitium.
  • Demonstrated automated, rapid, and reproducible low-cost manufacturing of the chip platform.

Conclusions:

  • The developed 3D-printed kidney tubulointerstitium-on-a-chip is a significant advancement for in vitro disease modeling.
  • This platform offers a promising tool for accelerating drug testing and development for chronic kidney disease.
  • Represents a foundational step towards creating more complex and functional kidney-on-a-chip systems.