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Related Concept Videos

Sample Preparation for Analysis: Advanced Techniques01:08

Sample Preparation for Analysis: Advanced Techniques

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Accurate analysis of complex samples often requires advanced preparation techniques to achieve reliable and reproducible results. Samples containing inorganic or organic materials can be challenging to dissolve or decompose effectively. Standard sample preparation methods include acid digestion, fusion, dry ashing, and wet digestion.
Acid digestion with strong acids is commonly used to dissolve inorganic materials that are insoluble (do not dissolve) in water. This method can be useful for...
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DNA Isolation01:24

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DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
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Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization
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Chemical Trends in Sample Preparation for Nucleic Acid Amplification Testing (NAAT): A Review.

Soo Min Lee1, Hari Kalathil Balakrishnan2, Egan H Doeven3

  • 1Centre for Regional and Rural Futures (CeRRF), Deakin University, Locked Bag 20000, Geelong, VIC 3220, Australia.

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|November 24, 2023
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Summary

Nucleic acid amplification testing requires effective sample preparation for reliable point-of-need testing (PONT). This review focuses on lysis and extraction reagents crucial for sensitive detection and minimizing inhibition in portable diagnostic formats.

Keywords:
NA extractioncell lysisnucleic acid amplification testingpoint-of-need testingsample preparationsolid-phase extraction

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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Analytical Chemistry

Background:

  • Nucleic acid amplification testing (NAAT) is vital for disease detection and early response to biological threats.
  • Effective sample preparation is critical for NAAT sensitivity and reliability, especially for point-of-need testing (PONT).
  • Challenges include inhibitor sensitivity and minimizing liquid handling for portable PONT applications.

Purpose of the Study:

  • To review reagents used in nucleic acid (NA) sample preparation for NAAT.
  • To assess the suitability of lysis and extraction reagents for PONT applications.
  • To highlight strategies for overcoming challenges in NAAT sample preparation.

Main Methods:

  • Literature review of NA lysis and extraction reagents.
  • Analysis of reagent performance in the context of PONT requirements.
  • Focus on binding, washing, and elution steps in NA extraction.

Main Results:

  • Sample preparation is a critical bottleneck in NAAT, impacting sensitivity and reliability.
  • Reagents must be carefully selected to avoid inhibitors and facilitate efficient NA extraction.
  • Minimizing liquid handling is key for developing portable PONT assays.

Conclusions:

  • Optimized sample preparation is essential for successful PONT NAAT.
  • Further development of reagents suitable for integrated, low-volume sample preparation is needed.
  • Addressing inhibitor carryover and extraction efficiency is paramount for robust PONT diagnostics.