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Continuous Real-Time Detection of Serotonin Using an Aptamer-Based Electrochemical Biosensor
Habib M N Ahmad1, Arturo Andrade2,3, Edward Song1
1Department of Electrical & Computer Engineering, University of New Hampshire, Durham, NH 03824, USA.
Biosensors
|November 24, 2023
Summary
This study introduces a novel electrochemical biosensor for continuous, real-time monitoring of serotonin (5-HT). The highly sensitive and selective aptamer-based sensor offers rapid detection, crucial for understanding neurological functions and mental health.
Area of Science:
- Neuroscience
- Biomedical Engineering
- Analytical Chemistry
Background:
- Serotonin (5-HT) is vital for neuronal functions, but its depletion is linked to mental health disorders.
- Detecting serotonin is challenging due to its rapid release/clearance, low concentrations, and interfering substances.
Purpose of the Study:
- To develop a highly sensitive and selective electrochemical aptamer-based biosensing platform for continuous serotonin monitoring.
- To enable real-time detection of serotonin in biological samples.
Main Methods:
- Utilized an electrochemical aptamer-based biosensor.
- Employed a single-frequency electrochemical impedance spectroscopy (EIS) technique for continuous monitoring.
- Tested sensor performance in continuous sample fluid flow.
Main Results:
- Achieved a response time of approximately 1 minute to concentration changes.
- Demonstrated a detection limit (LOD) of 5.6 nM for serotonin in the 25-150 nM range.
- Exhibited high selectivity against interfering compounds like dopamine and norepinephrine.
Conclusions:
- The developed aptasensor provides a robust platform for continuous, real-time serotonin detection with high sensitivity and selectivity.
- This technology holds potential for use in organ-on-a-chip and brain-on-a-chip systems for neurological research.
