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Membrane Cascade Fractionation of Tomato Leaf Extracts-Towards Bio-Based Crop Protection.

Emmanouil H Papaioannou1, Fabio Bazzarelli2, Rosalinda Mazzei2

  • 1School of Engineering, Lancaster University, Lancaster LA1 4YW, UK.

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Summary

Tomato leaf extracts, when fractionated using membranes, show significant crop protection activity. This efficient process isolates active compounds, primarily sugars, for enhanced disease resistance in plants.

Keywords:
bioactive compoundsbiorefinerymembrane processesplant diseasetomato leaves

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Area of Science:

  • Agricultural Science
  • Biotechnology
  • Plant Pathology

Background:

  • Initial studies indicated potential for tomato leaf extracts as crop protection agents.
  • Further research is needed to optimize extraction and identify active components.

Purpose of the Study:

  • To develop an efficient membrane-based pipeline for fractionating tomato leaf extracts.
  • To identify fractions with significant crop protection and disease resistance elicitor activity.

Main Methods:

  • Utilized ultrafiltration (UF) and nanofiltration (NF) membranes with varying molecular weight cut-offs (150 kDa, 10 kDa, 200-800 Da).
  • Analyzed changes in sugar, protein, and biophenol composition across fractions.
  • Evaluated crop protection activity of different membrane fractions.

Main Results:

  • A membrane cascade (UF 150 kDa, UF 10 kDa, NF 800 Da) efficiently separated tomato leaf extracts.
  • The fraction retained by the 800 Da NF membrane exhibited the highest crop protection activity.
  • This active fraction showed an increased ratio of sugars relative to proteins and biophenols, suggesting sugars are key active components.

Conclusions:

  • An optimized membrane filtration process can produce a potent disease resistance elicitor from tomato leaves.
  • The process is simple, chemical-free, and suitable for large-scale bio-pesticide formulation.
  • Remaining fractions can be utilized in a biorefinery, promoting sustainable agriculture.