Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Gas Chromatography–Mass Spectrometry (GC–MS)01:14

Gas Chromatography–Mass Spectrometry (GC–MS)

4.3K
Gas chromatography–mass spectrometry (GC–MS) is the combination of analytical techniques of gas chromatography and mass spectrometry in a single instrument for analyzing a mixture of compounds. The gas chromatograph separates the compounds in the mixture, and the mass spectrometer analyzes each compound separately to determine the molecular masses and molecular structures.
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall....
4.3K
Gas Chromatography: Introduction01:13

Gas Chromatography: Introduction

2.0K
Gas chromatography (GC) is a technique for separating and analyzing volatile compounds in a sample. Its primary purpose is to identify and quantify components in complex mixtures, making it essential in fields such as environmental analysis, pharmaceuticals, and petrochemicals. GC is also called vapor-phase chromatography (VPC) or gas-liquid partition chromatography (GLPC).
In GC,  a sample is vaporized and mixed with an inert carrier gas (the mobile phase), which transports it through a...
2.0K
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

793
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
793
Gas Chromatography: Types of Columns and Stationary Phases01:17

Gas Chromatography: Types of Columns and Stationary Phases

677
Gas chromatography (GC) relies on stationary phases to separate and analyze components in a sample. There are two main types of stationary phases: liquid and solid. Liquid stationary phases are non-volatile, thermally stable, and chemically inert liquids coated onto the column. Solid stationary phases are particles of adsorbent material, such as silica gel or molecular sieves.
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
677
Gas Chromatography: Overview of Detectors01:13

Gas Chromatography: Overview of Detectors

572
Detectors in gas chromatography (GC) help identify and quantify the components of a mixture by translating chemical properties into measurable signals, which are displayed on a chromatogram. Detectors can be categorized into two main types: destructive and non-destructive.
A non-destructive detector allows a sample to be analyzed without altering or consuming it, meaning the sample can be collected after detection for further analysis. Examples include thermal conductivity detectors and...
572
Supercritical Fluid Chromatography01:18

Supercritical Fluid Chromatography

258
Supercritical fluid chromatography (SFC) provides a beneficial substitute for gas chromatography (GC) and liquid chromatography (LC) for certain samples because it merges the top attributes of both techniques. SFC allows the separation and analysis of compounds that GC or LC does not easily manage. These compounds are traditionally nonvolatile or thermally unstable, making GC unsuitable and lacking functional groups required for HPLC analysis.
SFC utilizes a supercritical fluid mobile phase,...
258

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Valorization of Organic Food Surpluses and Brewer's Spent Grains into Organic Insect Protein for Replacing Soybean in Post-Weaning Piglets.

Insects·2026
Same author

Food and environment: advances in separation and sensing methodologies.

Analytical and bioanalytical chemistry·2026
Same author

Vacuum-assisted HS-SPME/GC×GC-MS to enhance the volatile chromatographic fingerprint of commercial dark chocolate.

Analytica chimica acta·2026
Same author

Update on the microwave-assisted saponification conditions for mineral oil hydrocarbons determination in fats and oils.

The Analyst·2025
Same author

Optimization and Validation of a Cheaper, Safer, and More Sustainable Methodology for Aflatoxins Determination in Rich-Lipidic Matrices (Pistachio Nuts) Using Deep Eutectic Solvent Extraction and UHPLC-FLD Analysis.

Journal of agricultural and food chemistry·2024
Same author

Changes in the fatty acid profiles and health indexes of bovine colostrum during the first days of lactation and their impact on human health.

Food chemistry·2024
Same journal

Analyses of dextroamphetamine and its metabolites in human urine by capillary electrophoresis with diode array and capacitively coupled contactless conductivity detection (CE-DAD-C<sup>4</sup>D).

Analytical and bioanalytical chemistry·2026
Same journal

Whole-body mass spectrometry imaging reveals metabolome and lipid peroxidation heterogeneity in zebrafish xenografts of esophageal squamous cell carcinoma.

Analytical and bioanalytical chemistry·2026
Same journal

A robust and validated method for the determination of 21 urinary metabolites of 15 plasticizers, including phthalates, DEHTP, and DINCH, by online SPE and liquid chromatography-tandem mass spectrometry.

Analytical and bioanalytical chemistry·2026
Same journal

A label-free membrane-based biosensor array with AuNP-modified PDMS for sensitive and specific detection of alpha-fetoprotein.

Analytical and bioanalytical chemistry·2026
Same journal

Smartphone-integrated one-step colorimetric glucose detection at physiological pH enabled by a haloperoxidase mimic.

Analytical and bioanalytical chemistry·2026
Same journal

Chemiluminescence functionalized magnetic nanoparticles-based biosensor for sensitive detection of glucose, uric acid, and cholesterol.

Analytical and bioanalytical chemistry·2026
See all related articles

Related Experiment Video

Updated: Jul 10, 2025

Gas Chromatography-Mass Spectrometry Paired with Total Vaporization Solid-Phase Microextraction as a Forensic Tool
05:31

Gas Chromatography-Mass Spectrometry Paired with Total Vaporization Solid-Phase Microextraction as a Forensic Tool

Published on: May 25, 2021

7.1K

Solid-phase microextraction coupled to comprehensive multidimensional gas chromatography for food analysis.

Juan Aspromonte1, Steven Mascrez2, Damien Eggermont2

  • 1Laboratorio de Investigación y Desarrollo de Métodos Analíticos, LIDMA, Facultad de Ciencias Exactas (Universidad Nacional de La Plata, CIC-PBA, CONICET), Calle 47 Esq. 115, 1900, La Plata, Argentina.

Analytical and Bioanalytical Chemistry
|November 24, 2023
PubMed
Summary
This summary is machine-generated.

Solid-phase microextraction (SPME) coupled with comprehensive two-dimensional gas chromatography (GC×GC) revolutionizes food analysis. This powerful technique enables a holistic approach to foodomics, moving beyond targeted analysis for deeper quality and authenticity insights.

Keywords:
FoodGC × GCMultidimensional comprehensive gas chromatographySPMESolid-phase microextraction

More Related Videos

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
10:14

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

Published on: September 2, 2020

5.0K
PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis
08:43

PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis

Published on: May 11, 2017

12.4K

Related Experiment Videos

Last Updated: Jul 10, 2025

Gas Chromatography-Mass Spectrometry Paired with Total Vaporization Solid-Phase Microextraction as a Forensic Tool
05:31

Gas Chromatography-Mass Spectrometry Paired with Total Vaporization Solid-Phase Microextraction as a Forensic Tool

Published on: May 25, 2021

7.1K
Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
10:14

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

Published on: September 2, 2020

5.0K
PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis
08:43

PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis

Published on: May 11, 2017

12.4K

Area of Science:

  • Separation Science
  • Analytical Chemistry
  • Food Science

Background:

  • Solid-phase microextraction (SPME) and comprehensive multidimensional gas chromatography (GC×GC) are key innovations from the 1990s.
  • Their combined application has significantly advanced analytical capabilities.

Purpose of the Study:

  • To critically review the applications of SPME-GC×GC-MS in food analysis.
  • To highlight the shift from targeted analysis to a holistic foodomics approach.
  • To emphasize the importance of data treatment for accurate interpretation.

Main Methods:

  • The review focuses on the coupling of Solid-phase microextraction (SPME) with comprehensive multidimensional gas chromatography (GC×GC).
  • Mass spectrometry (MS) is utilized for analyte detection and identification.
  • Applications in food analysis are critically examined.

Main Results:

  • SPME-GC×GC-MS enables comprehensive analysis of volatile and semi-volatile compounds in food.
  • This technique facilitates a holistic approach to foodomics, assessing quality and authenticity.
  • The coupling provides detailed insights into complex food matrices.

Conclusions:

  • The SPME-GC×GC-MS coupling is essential for modern food analysis and foodomics.
  • This methodology allows for a more sophisticated understanding of food quality and authenticity.
  • Appropriate data processing is crucial for deriving meaningful conclusions from complex datasets.