Related Experiment Video
Updated: Jul 10, 2025
Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans
Published on: February 7, 2018
An aggregation-induced emission-based ratiometric fluorescent chemosensor for Hg(II) and its application in
K Ramki1, G Thiruppathi2, Selva Kumar Ramasamy3
1Department of Nanoscience and Technology, Bharathiar University, Coimbatore 641 046, Tamil Nadu, India; Department of Immunology, School of Biological Sciences, Madurai Kamaraj University, Madurai 625 021, Tamil Nadu, India.
Abstract:
A chromone-based ratiometric fluorescent probe L2 was developed for the selective detection of Hg(II) in a semi-aqueous solution based on aggregation-induced emission (AIE) and chelation-enhanced fluorescence (CHEF) effect. The probe L2 fluoresced significantly at 498 nm in its aggregated state, and when chelated with Hg(II), the soluble state fluoresced 1-fold higher. In addition, Job's plot reveals that the probe forms a 1:1 stoichiometry complex with Hg(II) with an association constant of 9.10 × 103M-1 estimated by the BH plot. The probe L2 detects Hg(II) down to 22.47 nM without interference from other interfering ions. The FTIR, ESI mass, and DFT-based computational studies investigated the binding mechanism of probe L2 with Hg(II). Taking advantage of its AIE characteristics, the probe L2 was successfully applied for bio-capability analysis in Caenorhabditis elegans (a nematode worm) imaging of Hg(II) in a living model.
