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Co-Culturing Microalgae with Roseobacter Clade Bacteria as a Strategy for Vibrionaceae Control in Microalgae-Enriched
José Pintado1, Patricia Ruiz1, Gonzalo Del Olmo1
1Marine Ecology and Resources Group, Institute of Marine Research (IIM-CSIC), 36208 Vigo, Spain.
Microorganisms
|November 25, 2023
Summary
Introducing Roseobacter clade probiotics with microalgae effectively reduced Vibrionaceae in Artemia, preventing harmful bacteria from reaching fish larvae. This enhances aquaculture health and larval survival rates.
Area of Science:
- Marine microbiology
- Aquaculture science
- Probiotics in aquaculture
Background:
- Fish larval microbiota is influenced by live prey (rotifers, Artemia).
- Live prey microbiota is often dominated by detrimental, fast-growing Vibrionaceae.
- Opportunistic or pathogenic bacteria in feed can harm fish larvae.
Purpose of the Study:
- To investigate the co-cultivation of microalgae with Vibrio-antagonistic probiotics.
- To assess the impact of probiotics on microalgae growth and associated bacterial communities.
- To evaluate the efficacy of probiotic-enriched microalgae in reducing Vibrionaceae in Artemia.
Main Methods:
- Co-cultivation of microalgae (Phaeodactylum tricornutum, Chlorella minutissima) with Roseobacter clade probiotics (Phaeobacter spp., Ruegeria spp.).
- DGGE analysis to assess bacterial community composition.
- Scale-up of microalgae cultures with Ruegeria ALR6.
- Enrichment of Artemia with probiotic-inoculated microalgae.
Main Results:
- Probiotic introduction did not affect microalgae growth or bacterial community composition.
- Ruegeria ALR6 was successfully maintained in scaled-up microalgae cultures.
- Enrichment of Artemia with Ruegeria-inoculated P. tricornutum reduced Vibrionaceae by 2 Log units.
Conclusions:
- Co-cultivation of microalgae with Roseobacter probiotics is a viable strategy.
- This method effectively reduces Vibrionaceae in live feed (Artemia).
- Probiotic-enhanced microalgae can prevent the transfer of detrimental bacteria to fish larvae, improving aquaculture outcomes.
Keywords:
ArtemiaChlorella minutissimaPhaeobacterPhaeodactylum tricornutumRuegeriaVibrionaceaevibriosis controlMore Related Videos
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