A Ratiometric Fluorescent Probe for N₂H₄ Having a Large Detection Range Based upon Coumarin with Multiple...

Area of Science:

• Chemical Sensing
• Fluorescent Probes
• Analytical Chemistry

Background:

• Hydrazine (N2H4) is a widely used chemical with significant toxicity.
• Environmental and health risks necessitate effective monitoring of hydrazine.
• Existing detection methods may lack selectivity or sensitivity.

Purpose of the Study:

• To develop a novel ratiometric fluorescent probe for hydrazine detection.
• To synthesize and characterize a coumarin-based probe (CHAC) with an acetyl recognition group.
• To evaluate the probe's performance in various matrices and biological systems.

Main Methods:

• One-step synthesis of the coumarin-based fluorescent probe CHAC.
• Utilizing selective deprotection of the acetyl group by hydrazine to trigger a fluorescent response.
• Spectroscopic analysis to determine probe selectivity, sensitivity, and detection range.
• Application of the probe for detecting hydrazine in water, soil, gas, and living cells.

Main Results:

• CHAC exhibited high selectivity and anti-interference capabilities for hydrazine detection.
• Achieved a low limit of detection (0.16 μM) and a wide linear detection range (0-500 μM).
• Demonstrated effective hydrazine detection across a broad pH range (6-12).
• Successfully performed quantitative and qualitative hydrazine detection in environmental samples and living cells with low cytotoxicity.

Conclusions:

• The developed CHAC probe offers a robust and sensitive platform for hydrazine monitoring.
• Its ratiometric fluorescent response provides reliable quantitative detection.
• CHAC shows promise for real-world applications in environmental safety and biological imaging.