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Evaluation of the Efficacy of the H. pylori Protein HP-NAP as a Therapeutic Tool for Treatment of Bladder Cancer in an Orthotopic Murine Model

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An Evaluation of Urease A Subunit Nanocapsules as a Vaccine in a Mouse Model of Helicobacter pylori Infection.

Ivana Skakic¹, Jasmine E Francis¹, Chaitali Dekiwadia²

¹School of Science, RMIT University, 264 Plenty Road, Bundoora, VIC 3083, Australia.

|November 25, 2023

Summary

Protein nanocapsules were synthesized using silica templates to create potential vaccines against Helicobacter pylori. Larger nanocapsules, when combined with an adjuvant, significantly reduced bacterial colonization in mice.

Keywords:

Helicobacter pylori nanocapsule silica nanoparticle template urease alpha subunit vaccination

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Mouse Models Of Helicobacter Infection And Gastric Pathologies

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Published on: October 18, 2018

Area of Science:

Nanotechnology
Vaccine Development
Microbiology

Background:

Helicobacter pylori infection is a significant global health concern.
Developing effective vaccines against H. pylori remains a challenge.
Protein-based nanostructures offer a promising platform for vaccine delivery.

Purpose of the Study:

To synthesize and characterize protein nanocapsules using silica templates for potential H. pylori vaccines.
To evaluate the immunogenicity and protective efficacy of these nanocapsules in a mouse model.

Main Methods:

Synthesis of protein nanocapsules (UreA subunit of H. pylori urease) using two types of silica templates (SC/MS and MS) of different sizes.
Characterization of nanocapsule size, monodispersity, and homogeneity.
Vaccination of mice with different nanocapsule formulations and an adjuvant.
Assessment of immune responses and reduction in H. pylori colonization.

Main Results:

Two sizes of monodisperse protein nanocapsules (510 nm and 47 nm) were successfully synthesized.
Vaccination with larger nanocapsules (510 nm) combined with an adjuvant significantly reduced H. pylori colonization in mice.
Immune responses were evaluated, indicating the potential efficacy of the nanocapsule vaccine.

Conclusions:

Protein nanocapsules, particularly larger ones formulated with an adjuvant, show promise as a vaccine strategy against H. pylori infection.
This study demonstrates a novel approach for developing subunit vaccines using nanotechnology.
Further research is warranted to optimize the formulation and assess long-term efficacy.