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Projection Micro-Stereolithography to Manufacture a Biocompatible Micro-Optofluidic Device for Cell Concentration

Lorena Saitta1, Emanuela Cutuli2, Giovanni Celano1

  • 1Department of Civil Engineering and Architecture, University of Catania, Via Santa Sofia 64, 95125 Catania, Italy.

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Summary

A novel 3D printed biocompatible micro-optofluidic device enables precise monitoring of cell concentrations in biological fluids. This advancement utilizes Projection Microstereolithography (PμSL) for accurate two-phase flow analysis and optical detection.

Keywords:
3D printingcell concentration detectionmicro-opticsmicrofluidicsphotocurable biocompatible resinstwo-phase flow detectionvat photopolymerization

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Area of Science:

  • Biomedical Engineering
  • Optofluidics
  • Materials Science

Background:

  • Monitoring cell concentration in biological fluids is crucial for diagnostics and research.
  • Traditional methods can be complex and require specialized equipment.
  • Microfluidic devices offer miniaturization and precise control for fluid analysis.

Purpose of the Study:

  • To develop and validate a 3D printed biocompatible micro-optofluidic (MoF) device for two-phase flow monitoring.
  • To assess the performance of a novel biocompatible resin for optical detection applications.
  • To demonstrate the device's capability in monitoring biological fluid concentrations.

Main Methods:

  • Fabrication of the MoF device using Projection Microstereolithography (PμSL) with a novel biocompatible photocurable resin.
  • Integration of microfluidic channels and optical fiber slots for light signal acquisition.
  • Comparison of optical detection using biocompatible (BIO) resin versus non-biocompatible (HTL) resin.
  • Experimental validation with varying concentrations of Saccharomyces cerevisiae cells in PBS solution.

Main Results:

  • The 3D printed MoF device successfully controlled and monitored both air-water and cell-suspension two-phase flows.
  • The biocompatible resin demonstrated suitability for optical detection based on light absorption.
  • Optimized operating conditions (flow rate 0.1 mL/min, laser power 1-3 mW) enabled discrimination of cell concentrations.
  • High working ability (R2=0.9874, Radj2=0.9811) was achieved in monitoring yeast cell concentrations.

Conclusions:

  • 3D printed biocompatible micro-optofluidic devices are effective for monitoring cell concentrations in biological fluids.
  • The PμSL technique and novel biocompatible resin are suitable for creating integrated optofluidic systems.
  • The developed device offers a robust platform for real-time analysis of biological fluid properties.