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Cross-Platform Application of DNA Methylation Age Estimation Model in Eastern Chinese Han Population
Zi-Wei Wang1,2, Cheng-Tao Li1,2, Xi-Ling Liu2
1Department of Forensic Medicine, School of Basic Medicine and Biological Sciences, Suzhou Medical College of Soochow University, Suzhou 215123, China.
Objectives:
To evaluate the forensic application value of an age estimation model based on DNA methylation in eastern Chinese Han population, and to provide a theoretical basis for exploring age estimation models suitable for different detection platforms.
Methods:
According to the 6 age-related methylation sites in the published blood DNA methylation age estimation models of Chinese Han population, the DNA methylation level of 48 samples was detected by pyrosequencing and next-generation sequencing (NGS). After submitting DNA methylation levels to the age estimation model, the DNA methylation ages were predicted and compared with their real ages.
Results:
The 6 DNA methylation sites in both detection techniques were age-related, with an R2 of 0.85 and a median absolute deviation (MAD) of 4.81 years when using pyrosequencing；with an R2 of 0.84 and MAD of 4.41 years when using NGS.
Conclusions:
The blood DNA methylation age estimation model can be used under pyrosequencing and multi-purpose regional methylation enrichment sequencing technology based on NGS and it can accurately estimate the age.
