Bis-chalcone Fluorescent Probe for Hydrazine Ratio Sensing in Environment and Organism

In this paper, four novel hydrazine fluorescent probes X₁-X₄ with bis-chalcone structure were designed and synthesized. Through the measurement of its optical properties, it is found that it can quickly identify hydrazine, high sensitivity, low detection limit, and good anti-interference ability. The recognition of hydrazine by probes X₁-X₄ is not affected in the pH range of 4-10, X₂ has the highest sensitivity, and the detection limit is as low as 0.336 × 10^-7 M. Through Gaussian quantization calculation of probe molecules and their reaction products with hydrazine, it is speculated that the recognition mechanism is the closure of intramolecular charge transfer effect. In addition, the cytotoxicity and imaging of HeLa cells were tested, which showed that probes X₁-X₄ could be used to detect hydrazine in cells.