Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

BioPipelines: Accessible Computational Protein and Ligand Design for Chemical Biologists.

Computational and structural biotechnology journal·2026

Same author

A Ratiometric pH Sensor for Gram-Positive and Gram-Negative Bacteria.

Journal of the American Chemical Society·2026

Same author

Two-Color Timestamping of Gene Expression with a Chemigenetic Reporter System.

Chembiochem : a European journal of chemical biology·2025

Same author

Potent Inducers of Paraptosis through Electronic Tuning of Hemicyanine Electrophiles.

Journal of the American Chemical Society·2025

Same author

A Far-Red Fluorescent Probe to Visualize Gram-Positive Bacteria in Patient Samples.

ACS infectious diseases·2024

Same author

A locally activatable sensor for robust quantification of organellar glutathione.

Nature chemistry·2023

Same journal

A rechargeable non-aqueous Mg-O<sub>2</sub> battery based on magnesium peroxide chemistry.

Nature chemistry·2026

Same journal

Setting a direction for molecular motors.

Nature chemistry·2026

Same journal

Driving movement in the field of molecular machines.

Nature chemistry·2026

Same journal

First ladies of chemistry.

Nature chemistry·2026

Same journal

How isoprene connects plants to global climate.

Nature chemistry·2026

Same journal

One-dimensional carbon chains free of end-capping groups.

Nature chemistry·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jul 9, 2025

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Published on: July 6, 2016

A general strategy to develop fluorogenic polymethine dyes for bioimaging.

Annabell Martin^1,2, Pablo Rivera-Fuentes³

¹Department of Chemistry, University of Zurich, Zurich, Switzerland.

Nature Chemistry

|November 27, 2023

Summary

This summary is machine-generated.

Researchers developed a new method to create bright, fluorogenic dyes from polymethine compounds. These advanced probes offer improved imaging capabilities, emitting light across the visible and near-infrared spectrum for biological studies.

More Related Videos

Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy

Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy

Published on: June 4, 2020

Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors

Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors

Published on: September 10, 2013

Related Experiment Videos

Last Updated: Jul 9, 2025

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Published on: July 6, 2016

Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy

Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy

Published on: June 4, 2020

Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors

Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors

Published on: September 10, 2013

Area of Science:

Chemical Biology
Molecular Imaging
Organic Synthesis

Background:

Fluorescence imaging is crucial for studying biological processes.
Existing fluorogenic rhodamine dyes have limitations, including complex synthesis and spectral range.

Purpose of the Study:

To develop a general strategy for creating novel fluorogenic dyes.
To overcome spectral limitations of current rhodamine dyes.
To create probes with enhanced imaging properties.

Main Methods:

A general strategy transforming polymethine compounds into fluorogenic dyes via intramolecular ring-closure.
Synthesis of spontaneously blinking and no-wash, turn-on polymethine dyes.
Demonstration of probes' compatibility with self-labelling proteins and targeting ligands.

Main Results:

Successfully created polymethine-based fluorogenic dyes with emissions across visible and near-infrared spectra.
Developed probes exhibiting improved brightness and red-shifted emission compared to rhodamine dyes.
Demonstrated multicolour and fluorescence lifetime multiplexing imaging capabilities.

Conclusions:

The intramolecular ring-closure strategy provides access to versatile, bright fluorogenic dyes.
These novel dyes expand the spectral range for fluorescence imaging, particularly into the near-infrared.
The developed probes offer significant advantages for advanced biological imaging applications.