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Reporter Genes

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Updated: Jan 18, 2026

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

Two-Color Timestamping of Gene Expression with a Chemigenetic Reporter System.

Henriette Lämmermann¹, Jade Nguyen², Juan F Tamez-Fernández¹

¹Department of Chemistry, University of Zurich, 8057, Zurich, Switzerland.

Chembiochem : a European Journal of Chemical Biology

|September 12, 2025

Summary

This summary is machine-generated.

Researchers developed a new reporter assay to monitor BiP/GRP78 expression, a key part of the unfolded protein response. This tool identified a novel activator of BiP/GRP78, aiding stress response research.

Keywords:

BiP/GRP78 HaloTag high‐content screening reporter gene assays

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Published on: July 30, 2020

Area of Science:

Cellular Biology
Molecular Biology
Biochemistry

Background:

Stress response pathways are crucial for cellular homeostasis.
The unfolded protein response (UPR) in the endoplasmic reticulum maintains protein folding and processing.
BiP/GRP78 is a key regulator of the UPR, and monitoring its expression is vital.

Purpose of the Study:

To develop a novel chemigenetic reporter assay for monitoring BiP/GRP78 expression.
To enable flexible and sensitive detection of BiP/GRP78 levels in live cells.
To identify novel activators of BiP/GRP78 expression through high-content screening.

Main Methods:

Development of a reporter system utilizing coexpression of HaloTag via an internal ribosome entry site.
Implementation of a two-color timestamping strategy for enhanced readout sensitivity.
Application of the reporter system in live-cell imaging, flow cytometry, and high-content screening.

Main Results:

A functional chemigenetic reporter assay for BiP/GRP78 expression was successfully established.
The assay demonstrated flexibility in labeling and improved sensitivity through a two-color timestamping strategy.
A high-content screen utilizing the reporter system identified a novel and promising activator of BiP/GRP78 expression.

Conclusions:

The developed reporter system offers a versatile and sensitive tool for studying the unfolded protein response.
This assay facilitates the discovery of new modulators of cellular stress pathways.
The identified BiP/GRP78 activator holds potential for therapeutic applications in protein folding-related diseases.