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Related Concept Videos

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
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Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...

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Updated: May 11, 2026

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
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A HaloTag-Based Reporter System Enabling Two-Color Timestamped Gene Expression Monitoring.

Henriette Lämmermann1

  • 1Department of Chemistry, University of Zurich, CH-8057 Zurich. henriette.laemmermann@chem.uzh.ch.

Chimia
|May 9, 2026
PubMed
Summary
This summary is machine-generated.

Researchers created a new reporter system to track BiP/GRP78 gene expression. This system identified a novel activator of the unfolded protein response (UPRER), crucial for cellular homeostasis during stress.

Keywords:
BiP/GRP78HaloTagHigh-content screeningReporter gene assay

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Area of Science:

  • Molecular Biology
  • Cellular Biology
  • Biochemistry

Background:

  • Precise gene expression regulation is vital for cellular homeostasis and stress responses.
  • The unfolded protein response (UPRER) in the endoplasmic reticulum maintains protein folding capacity.
  • BiP/GRP78 is a key regulator within the UPRER pathway.

Purpose of the Study:

  • To develop a novel chemigenetic reporter system for monitoring BiP/GRP78 gene expression.
  • To enhance readout sensitivity using a two-color sequential labeling strategy.
  • To identify novel activators of BiP/GRP78 expression through high-content screening.

Main Methods:

  • Development of a chemigenetic reporter system utilizing co-expressed HaloTag protein.
  • Implementation of a two-color sequential labeling technique for improved sensitivity.
  • Application of the reporter system in a high-content screening assay.

Main Results:

  • Successful development of a sensitive chemigenetic reporter for BiP/GRP78.
  • Identification of a novel compound that activates BiP/GRP78 expression.
  • Demonstration of the system's utility in discovering modulators of cellular stress response.

Conclusions:

  • The developed reporter system provides a sensitive tool for studying BiP/GRP78 regulation.
  • This system facilitates the discovery of new therapeutic targets for protein-folding diseases.
  • The findings highlight the importance of UPRER in cellular homeostasis and stress adaptation.