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Updated: Jul 9, 2025

Deep-Tissue Three-Photon Fluorescence Microscopy in Intact Mouse and Zebrafish Brain

Deep-Tissue Three-Photon Fluorescence Microscopy in Intact Mouse and Zebrafish Brain

Published on: January 13, 2022

A Large Field-of-view, Single-cell-resolution Two- and Three-Photon Microscope for Deep Imaging.

Aaron T Mok^1,2, Tianyu Wang¹, Shitong Zhao¹

¹School of Applied Engineering Physics, Cornell University, NY, USA.

Biorxiv : the Preprint Server for Biology

|November 28, 2023

Summary

This summary is machine-generated.

Researchers developed new three-photon microscopy techniques to overcome field-of-view limitations for deep brain imaging. This allows unprecedented large-scale, high-speed neuronal activity mapping in scattering tissues.

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Area of Science:

Neuroscience
Biomedical Engineering
Optical Imaging

Background:

In vivo imaging of neural networks is crucial for understanding brain function.
Multiphoton microscopy advances speed, field of view, and depth but faces limitations in scattering tissues.
Field of view in deep-tissue imaging decreases exponentially with depth due to thermal damage concerns.

Conclusions:

The developed innovations overcome limitations in deep-tissue multiphoton imaging.
These techniques facilitate large-field-of-view, system-level neural circuit research.
The methods are adaptable to existing multiphoton microscopes.