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Updated: Jul 9, 2025
08:25
Identification of Circular RNAs using RNA Sequencing
Published on: November 14, 2019
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ESRP1 controls biogenesis and function of a large abundant multiexon circRNA
Dawei Liu1, B Kate Dredge1, Andrew G Bert1
1Centre for Cancer Biology, SA Pathology and University of South Australia, Adelaide, SA 5000, Australia.
Nucleic Acids Research
|November 28, 2023
Summary
A novel circular RNA, circDOCK1(2-27), is highly abundant and regulated during epithelial-mesenchymal transition (EMT). Its formation, controlled by ESRP1, represses cell motility by altering DOCK1 mRNA production and inhibiting migration.
Area of Science:
- Molecular Biology
- RNA Biology
- Cell Biology
Background:
- Circular RNAs (circRNAs) are typically formed by rare back-splicing events.
- Some circRNAs are abundant and regulated, playing significant roles in cellular processes.
Purpose of the Study:
- To investigate the regulation, biogenesis, and function of circDOCK1(2-27).
- To elucidate the role of ESRP1 in circRNA formation and its impact on epithelial-mesenchymal transition (EMT).
Main Methods:
- HITS-CLIP analysis
- CRISPR-mediated deletions
- Proximity-dependent biotinylation (BioID) assay
- X-ray crystallography
Main Results:
- circDOCK1(2-27) is abundant, highly regulated during EMT, and its formation depends on ESRP1.
- circDOCK1(2-27) synthesis represses cell motility by diverting DOCK1 mRNA production and directly inhibiting migration.
- ESRP1 binds a GGU-repeat in intron 1, regulating circDOCK1(2-27) biosynthesis.
- ESRP1's structure reveals binding to GGU motifs via aromatic pockets.
Conclusions:
- circDOCK1(2-27) is a key regulator of cell motility during EMT.
- ESRP1 controls circDOCK1(2-27) biogenesis through specific RNA-binding interactions.
- Understanding circRNA regulation provides insights into cancer progression and therapeutic strategies.
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