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Updated: Jul 9, 2025

3' End Sequencing Library Preparation with A-seq2
Published on: October 10, 2017
selSeq: A method for the enrichment of non-polyadenylated RNAs including enhancer and long non-coding RNAs for
Jason D Limberis1, Alina Nalyvayko1, Joel D Ernst1
1Division of Experimental Medicine, University of California, San Francisco, San Francisco, CA, United States of America.
Abstract:
Non-polyadenylated RNA includes a large subset of crucial regulators of RNA expression and constitutes a substantial portion of the transcriptome, playing essential roles in gene regulation. For example, enhancer RNAs are long non-coding RNAs that perform enhancer-like functions, are bi-directionally transcribed, and usually lack polyA tails. This paper presents a novel method, selSeq, that selectively removes mRNA and pre-mRNA from samples enabling the selective sequencing of crucial regulatory elements, including non-polyadenylated RNAs such as long non-coding RNA, enhancer RNA, and non-canonical mRNA.
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