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Related Concept Videos

Colloidal precipitates01:09

Colloidal precipitates

The high insolubility of some precipitates can result in an unfavorable relative supersaturation. This can lead to colloidal particles with a large surface-to-mass ratio, where adsorption is promoted. For instance, in the precipitation of silver chloride, silver ions are adsorbed on the surface of the colloidal particles, forming a primary layer. This layer attracts ions of opposite charge (such as nitrate ions), forming a diffuse secondary layer of adsorbed ions. This electric double layer...

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Related Experiment Video

Updated: Jun 16, 2026

A Modified Precipitation Method to Isolate Urinary Exosomes
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Exosome Precipitation by Ionic Strength Modulation: ExoPRISM.

Vijaya Sunkara1, Juhee Park1, Jiyun Han1,2

  • 1Center for Soft and Living Matter, Institute for Basic Science (IBS), Ulsan 44919, Republic of Korea.

ACS Applied Materials & Interfaces
|November 28, 2023
PubMed
Summary
This summary is machine-generated.

We developed ExoPRISM, a simple, low-cost method to efficiently isolate extracellular vesicles (EVs) from biological fluids. This technique offers high yields and purity, advancing liquid biopsies and regenerative medicine.

Keywords:
EV isolationEV precipitationEV separationelectrolytesexosomesextracellular vesicles (EV)

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Area of Science:

  • Biochemistry
  • Biotechnology
  • Nanotechnology

Background:

  • Extracellular vesicles (EVs) are vital for theranostics but challenging to isolate.
  • Current EV separation methods are inefficient, costly, and not scalable.
  • Improved EV isolation is critical for advancing diagnostics and therapeutics.

Purpose of the Study:

  • To present ExoPRISM, a novel, cost-effective, and user-friendly method for EV separation.
  • To demonstrate high yield and purity of EVs isolated using ExoPRISM.
  • To validate ExoPRISM's adaptability across various biological fluid types.

Main Methods:

  • EV precipitation by ionic strength modulation (ExoPRISM) was developed.
  • Sequential precipitation of proteins and EVs using incremental electrolyte addition.
  • Low-speed centrifugation and washing for fractional EV separation.
  • Process completion in under one hour.

Main Results:

  • ExoPRISM achieves high yields and purity of EVs.
  • Isolated EVs retain their biological functions.
  • The method is effective for diverse biological fluids (plasma, serum, urine, culture medium).
  • ExoPRISM is scalable and cost-effective.

Conclusions:

  • ExoPRISM offers a superior alternative to existing EV separation techniques.
  • This method supports the development of next-generation liquid biopsies.
  • ExoPRISM shows significant potential for regenerative medicine applications.