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Related Experiment Video

Updated: Jul 9, 2025

Author Spotlight: Exploring Cell Migration and Gene Roles in the Developing Brain
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Single-cell analysis reveals specific neuronal transition during mouse corticogenesis.

Ziheng Zhou1,2,3, Yueyang Pan4, Si Zhou2

  • 1Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China.

Frontiers in Cell and Developmental Biology
|November 29, 2023
PubMed
Summary

Researchers discovered a direct transition from deep- to upper-layer neurons (DLNs to ULNs) during mouse fetal brain development. This cell population, crucial for neuronal migration, may explain human-mouse cortical development differences.

Keywords:
cerebral cortex developmentdeep-layer neuronsneuronal migrationneuronal transitionsingle-cell RNA sequencingupper-layer neurons

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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Genomics

Background:

  • Corticogenesis mechanisms remain incompletely understood.
  • Single-cell RNA sequencing (scRNA-seq) offers a powerful tool for dissecting developmental processes.
  • Fetal cortical development involves complex cellular transitions and gene expression dynamics.

Purpose of the Study:

  • To characterize the cellular and molecular mechanisms of corticogenesis.
  • To identify novel cell populations and developmental trajectories in the fetal cortex.
  • To investigate potential differences in cortical development between mice and humans.

Main Methods:

  • Comprehensive analysis of single-cell RNA sequencing (scRNA-seq) datasets from mouse and human fetal cortex.
  • Identification and characterization of specific cell populations and their developmental trajectories.
  • Validation of findings using co-immunostaining experiments across different developmental timepoints.

Main Results:

  • A distinct cell population exhibiting direct transition from deep-layer neurons (DLNs) to upper-layer neurons (ULNs) was identified in the developing mouse cortex (E13.5).
  • Genes highly expressed in this transitional population were enriched in pathways related to cell migration (e.g., PTN/MDK signaling).
  • Six genes showed opposing temporal expression patterns between human and mouse fetal cortex, linked to neuronal migration and cognitive functions.

Conclusions:

  • A novel direct DLN-to-ULN neuronal transition (D-T-U) population was characterized during mouse fetal corticogenesis.
  • This D-T-U population plays a role in neuronal migration.
  • Observed differences in gene expression between species may underlie variations in cortical development.