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Related Experiment Video

Updated: Jul 9, 2025

A Microfluidic-based Hydrodynamic Trap for Single Particles
10:13

A Microfluidic-based Hydrodynamic Trap for Single Particles

Published on: January 21, 2011

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Single-cell trapping and retrieval in open microfluidics.

Tomoki Murakami1, Hiroto Teratani1, Dai'ichiro Aoki2

  • 1Department of Precision Mechanics, Graduate School of Science and Engineering, Chuo University, Kasuga 1-13-27, Bunkyo-ku, Tokyo 112-8551, Japan.

Iscience
|November 29, 2023
PubMed
Summary

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This study introduces an open microfluidics system for hydrodynamic cell trapping, enabling external access to captured single cells. This innovation allows for automated retrieval, advancing single-cell analysis capabilities.

Area of Science:

  • Microfluidics and Cell Biology
  • Biotechnology and Bioengineering

Background:

  • Hydrodynamic cell trapping systems are versatile single-cell analysis platforms.
  • Existing deterministic systems confine cells within closed microchannels, limiting external access for cell retrieval and manipulation.

Purpose of the Study:

  • To develop an open microfluidics hydrodynamic cell-trapping system.
  • To enable external access and retrieval of trapped single cells.

Main Methods:

  • Development of a technique to selectively render the interior of polydimethylsiloxane (PDMS) microchannels hydrophilic.
  • Utilizing spontaneous capillary flow within an open microchannel architecture for precise cell confinement.
  • Demonstration of single bead and single cell trapping and subsequent retrieval using automated robotic pipetting.
Keywords:
Applied sciencesCell biology

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Related Experiment Videos

Last Updated: Jul 9, 2025

A Microfluidic-based Hydrodynamic Trap for Single Particles
10:13

A Microfluidic-based Hydrodynamic Trap for Single Particles

Published on: January 21, 2011

16.7K
Cell Capture Using a Microfluidic Device
29:02

Cell Capture Using a Microfluidic Device

Published on: October 1, 2007

5.5K
A Microfluidic Platform for High-throughput Single-cell Isolation and Culture
09:51

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Published on: June 16, 2016

11.4K

Main Results:

  • Successful implementation of an open microfluidics system for hydrodynamic cell trapping.
  • Demonstrated precise confinement of capillary flow in microchannels as narrow as tens of micrometers.
  • Achieved efficient trapping and automated retrieval of single cells.

Conclusions:

  • The developed open hydrodynamic cell-trapping system overcomes limitations of closed systems.
  • This platform facilitates integration with macro-scale biological apparatus, enabling novel single-cell analytical systems.
  • The system enhances the versatility of microfluidic devices for advanced biological research.