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Citrate-based fluorometric sensor for multi-halide sensing.

Dingbowen Wang1, Tunan Xia2, Yuqi Wang1

  • 1Department of Biomedical Engineering, Materials Research Institute, The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, 16802, USA.

Smart Materials in Medicine
|November 30, 2023
PubMed
Summary
This summary is machine-generated.

This study introduces a novel fluorometric method for simultaneously detecting multiple halide ions using a single citrate-based fluorophore (CA-Cys). This approach offers a fast, low-cost solution for environmental monitoring, overcoming interference issues in current methods.

Keywords:
BromideChlorideFluorometricHalide sensingSmartphone-based device

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Area of Science:

  • Analytical Chemistry
  • Environmental Science
  • Materials Science

Background:

  • Halides are crucial in human health and environmental monitoring.
  • Existing halide measurement methods suffer from cross-interference between different halide types.
  • Simultaneous, rapid, and cost-effective detection of multiple halides remains a significant analytical challenge.

Purpose of the Study:

  • To develop a novel fluorometric sensing method for simultaneous detection of multiple halide ions.
  • To overcome the limitations of current methods, such as interference and cost.
  • To create a portable and low-cost device for real-world applications.

Main Methods:

  • Utilized a single citrate-based fluorophore, CA-Cys, for fluorescence quenching-based sensing.
  • Employed dynamic quenching mechanisms where fluorescence intensity varies with halide concentration and pH.
  • Developed a system to solve multiple Stern-Volmer equations at different pH values for individual halide quantification.
  • Fabricated and tested a proof-of-concept smartphone-based portable fluorometer.

Main Results:

  • Demonstrated a millimolar (mM) scale detection limit suitable for wastewater monitoring.
  • Achieved simultaneous sensing of multiple halides by analyzing variations in fluorescence quenching at different pH levels.
  • Validated the method's efficacy using both a custom-made fluorometer and a portable smartphone-based device.
  • Showcased the potential for accurate halide concentration determination through a system of equations.

Conclusions:

  • The developed CA-Cys fluorometric method enables simultaneous, fast, and low-cost multi-halide sensing.
  • The portable device offers a promising solution for real-world environmental monitoring of halides.
  • This work paves the way for new portable and cost-effective multi-halide detection technologies.