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Related Experiment Video

Updated: Jun 25, 2026

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Published on: March 9, 2017

Toward the Development of Simplified Lateral Flow Assays Using Hydrogels as the Universal Control Line.

Tao Ma¹, Linlin Peng¹, Qinying Ran¹

¹The State Key Laboratory of Refractories and Metallurgy, School of Chemistry and Chemical Engineering, Wuhan University of Science and Technology, Wuhan 430081, China.

ACS Applied Bio Materials

|November 30, 2023

Summary

This study introduces a simplified hydrogel line (HL) modification for lateral flow assays (LFA), enhancing sensitivity by 2-5 fold. This innovation streamlines LFA manufacturing and improves detection capabilities for point-of-care testing.

Keywords:

COVID-19 gold nanoparticles hydrogel lateral flow assays surface modification

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Area of Science:

Biomedical Engineering
Analytical Chemistry
Materials Science

Background:

Lateral flow assays (LFA) are vital point-of-care testing devices.
Current LFA manufacturing involves preprinting costly bioreceptors, and detection sensitivity is limited.
Complex manufacturing and limited detection capabilities hinder LFA application in challenging fields.

Purpose of the Study:

To propose a novel and simple strategy to simplify LFA manufacture.
To enhance the sensitivity of LFA by modifying the hydrogel line (HL).
To develop a universal control line for multiple LFA detection modes.

Main Methods:

Incorporation of an extra hydrogel line (HL) into commercial LFA strips.
Substitution of the conventional antibody control line with a hydrogel control line (HCL).
Investigation of HCL performance based on fluid slowdown and interception effects, influenced by hydrogel concentration, permeation, and hydrophilicity.

Main Results:

Sensitivity of commercial LFA strips was enhanced by 2-5 fold with the incorporation of an extra HL.
A universal hydrogel control line (HCL) was successfully developed, accommodating multiple LFA detection modes.
HCL performance was demonstrated to be dependent on hydrogel properties and concentration within the nitrocellulose membrane.

Conclusions:

The modified hydrogel line strategy simplifies LFA manufacturing.
The hydrogel control line enhances LFA sensitivity and offers a universal detection platform.
This approach lays the foundation for developing simplified and more sensitive LFA sensing platforms.