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Updated: Jul 9, 2025
Evaluation of Intracellular Location of Reactive Oxygen Species in Solea Senegalensis Spermatozoa
Published on: March 11, 2018
The protective effect of zinc oxide and selenium oxide nanoparticles on the functional parameters of rat sperm during
Nafiseh Tavakolpoor Saleh1, Zohreh Hosseinzadeh2, Narges Gholami Banadkuki3
1Depaetment of Biophysics and Biochemistry, Faculty of Advanced Science and Technology, Islamic Azad University Tehran Medical sciences, Tehran, Iran.
Objective:
While sperm freezing (cryopreservation) is an effective method for preserving fertility, it can potentially harm the structure and function of sperm due to an increase in the production of reactive oxygen species. This study aimed to assess the impact of zinc oxide nanoparticles (ZnONPs) and selenium oxide nanoparticles (SeONPs) on various sperm functional parameters, including motility, plasma membrane integrity (PMI), mitochondrial membrane potential (MMP), acrosome membrane integrity (ACi), and malondialdehyde (MDA) levels.
Methods:
Semen samples were collected from 20 Albino Wistar rats. These samples were then divided into six groups: fresh, cryopreservation control, and groups supplemented with SeONPs (1, 2, 5 μg/mL) and ZnONPs (0.1, 1, 10 μg/mL).
Results:
Statistical analysis revealed that all concentrations of SeONPs increased total motility and progressive reduction of MDA levels compared to the cryopreservation control group (p<0.05). However, supplementation with ZnONPs did not affect these parameters (p>0.05). Conversely, supplements of 1 and 2 μg/mL SeONPs and 1 μg/mL ZnONPs contributed to the improvement of PMI and ACi (p<0.05). Yet, no significant change was observed in MMP with any concentration of SeONPs and ZnONPs compared to the cryopreservation control group (p>0.05).
Conclusion:
The findings suggest that optimal concentrations of SeONPs may enhance sperm parameters during the freezing process.
