Related Experiment Video
Updated: Jul 9, 2025
10:37
Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice
Published on: May 2, 2025
168
Protocol to characterize basement membranes during kidney development using mass spectrometry-based label-free
Mychel R P T Morais1, Pinyuan Tian1, Ronan O'cualain1
1Wellcome Centre for Cell-Matrix Research, University of Manchester, Manchester M13 9PT, UK.
STAR Protocols
|December 1, 2023
Summary
This study introduces a mass spectrometry proteomics protocol to analyze basement membrane composition in developing human kidney organoids and mouse kidneys. This method aids in understanding extracellular matrix regulation and function.
Area of Science:
- Biochemistry
- Developmental Biology
- Proteomics
Background:
- Basement membranes are crucial extracellular matrices providing structural and signaling support.
- These matrices are dynamic and play vital roles during tissue development.
- Understanding basement membrane composition is key to studying tissue regulation.
Purpose of the Study:
- To present a novel mass spectrometry-based proteomics protocol for basement membrane analysis.
- To define the composition of basement membranes in human kidney organoids and mouse fetal kidneys.
- To facilitate the study of extracellular matrix components during development.
Main Methods:
- Label-free quantitative proteomics using mass spectrometry.
- Analysis of basement membrane and extracellular matrix components.
- Utilized samples from human kidney organoids and mouse fetal kidneys.
Main Results:
- A detailed protocol for basement membrane proteomics was established.
- The composition of basement membranes in developing kidneys was investigated.
- The protocol enables the study of matrix regulation.
Conclusions:
- The presented protocol is valuable for investigating basement membrane and ECM dynamics.
- This research enhances understanding of matrix regulation during development.
- The findings support further studies in developmental biology and regenerative medicine.
