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Related Experiment Video

Updated: Jul 9, 2025

Author Spotlight: Designing Simple and Inexpensive Techniques to Grow Methane-Oxidizing Bacteria in the Laboratory

Author Spotlight: Designing Simple and Inexpensive Techniques to Grow Methane-Oxidizing Bacteria in the Laboratory

Published on: September 6, 2024

Engineered Methylococcus capsulatus Bath for efficient methane conversion to isoprene.

Georgii Emelianov¹, Dong-Uk Song², Nulee Jang³

¹Synthetic Biology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea; Department of Biosystems and Bioengineering, KRIBB School of Biotechnology, University of Science & Technology (UST), Daejeon 34113, Republic of Korea.

Bioresource Technology

|December 1, 2023

Summary

This summary is machine-generated.

Engineered microbes can now produce isoprene from methane, a sustainable biofuel precursor. This breakthrough offers a cost-effective and eco-friendly method for producing valuable chemicals from natural gas.

Keywords:

Isoprene Methane valorization Methanotroph Mevalonate

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Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies

Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies

Published on: January 23, 2019

Area of Science:

Biotechnology
Synthetic Biology
Microbial Engineering

Background:

Isoprene is a valuable industrial chemical with applications in polymers and biofuels.
Current production methods have environmental drawbacks and high costs.
Microbial production from methane offers a sustainable and economical alternative.

Purpose of the Study:

To engineer Methylococcus capsulatus Bath for methane-based isoprene production.
To enhance isoprene yield and minimize product degradation.
To establish a scalable bioprocess for isoprene synthesis.

Main Methods:

Introduction of the exogenous mevalonate (MVA) pathway into M. capsulatus Bath.
Overexpression of MVA pathway enzymes and isoprene synthase using a phenol-inducible promoter.
CRISPR-base editing to disrupt soluble methane monooxygenase (sMMO) expression.
Optimization of metabolic flux and culture conditions.

Main Results:

Successful engineering of M. capsulatus Bath for isoprene production from methane.
Significant improvement in isoprene production through gene overexpression and sMMO disruption.
Achieved a record titer of 228.1 mg/L for methanotroph-based isoprene production.
Demonstrated reduced toxicity and enhanced product stability.

Conclusions:

Methane-based isoprene production using engineered methanotrophs is feasible and efficient.
This bioprocess offers a sustainable route for producing value-added chemicals.
The developed strain and process hold potential for industrial-scale bio-manufacturing.