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Updated: Jul 9, 2025
Imaging Through the Pupal Case of Drosophila melanogaster
Published on: January 23, 2014
A live-imaging protocol for tracking intestinal stem cell divisions in the Drosophila melanogaster pupal midgut
Song Wu1, Ruizhi Tang2, Benjamin Ohlstein3
1Department of Pharmacology, Bioengineering and Food College, Hubei University of Technology, Wuhan 430068, China; Department of Medical Genetics, School of Basic Medicine, Institute for Brain Research, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Abstract:
Establishing a long-term ex vivo observation of the intestinal stem cell (ISC) is crucial to help understand the formation and homeostasis of the intestinal epithelium. Here, we present a protocol for tracking the division of Drosophila pupal ISCs during pupal midgut development. We describe steps for dissecting, mounting, and live imaging the pupal midgut. We then detail procedures for fluorescence quantification of each cell. This protocol can be applied to other fluorescently tagged proteins. For complete details on the use and execution of this protocol, please refer to Wu et al.1.
