Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Enterocyte protein processing and synthesis.

D H Alpers

    Hepatology (Baltimore, Md.)
    |January 1, 1987
    PubMed
    Summary
    This summary is machine-generated.

    Researchers are advancing brush border protein studies using molecular biology techniques. New methods like cDNA cloning and cell-free translation offer deeper insights into protein synthesis and regulation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Assessing somatization in urologic chronic pelvic pain syndrome.

    BMC urology·2019
    Same author

    Production of ectopic gastric intrinsic factor in gastric mucosa of humans with chronic gastritis.

    Digestive diseases and sciences·2011
    Same author

    Postcrystallization Analysis of the Irreproducibility of the Human Intrinsic Factor-Cobalamin Complex Crystals.

    Crystal growth & design·2009
    Same author

    Development of drugs for gastrointestinal motor disorders: translating science to clinical need.

    Neurogastroenterology and motility·2008
    Same author

    Crystal structure of human intrinsic factor: cobalamin complex at 2.6-A resolution.

    Proceedings of the National Academy of Sciences of the United States of America·2007
    Same author

    Effect of the NK(3) receptor antagonist, talnetant, on rectal sensory function and compliance in healthy humans.

    Neurogastroenterology and motility·2007
    Same journal

    Decoding fibrosis: Transcriptomic and clinical insights via AI-derived collagen deposition phenotypes in MASLD.

    Hepatology (Baltimore, Md.)·2026
    Same journal

    A randomized controlled trial of stepped treatment to reduce unhealthy alcohol use in patients with chronic liver disease.

    Hepatology (Baltimore, Md.)·2026
    Same journal

    AASLD AST NASPGHAN Practice Guideline on pediatric liver transplantation: Candidate evaluation.

    Hepatology (Baltimore, Md.)·2026
    Same journal

    H4K12 lactylation drives TREM2high macrophages differentiation in liver fibrosis.

    Hepatology (Baltimore, Md.)·2026
    Same journal

    Good vibrations: Using VCTE to predict hepatic decompensation in MASLD.

    Hepatology (Baltimore, Md.)·2026
    Same journal

    One size does not fit all: Global variability in noninvasive testing for MASLD.

    Hepatology (Baltimore, Md.)·2026
    See all related articles

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Cell Biology

    Background:

    • Structural information on brush border proteins traditionally relied on protein purification and chemistry.
    • Protein synthesis regulation was primarily studied using inhibitors of protein or nucleic acid synthesis.

    Purpose of the Study:

    • To explore the evolution of methodologies for studying brush border proteins.
    • To highlight advancements in understanding protein synthesis and regulation.

    Main Methods:

    • Protein purification and chemistry.
    • Cell-free translation and cDNA cloning.
    • Assessment of mRNA content via cell-free translation or cDNA hybridization.
    • Polysomal isolation and organ explants for direct synthetic and secretion rate determination.

    Related Experiment Videos

    Main Results:

    • Molecular biology techniques have significantly expanded the ability to study brush border proteins.
    • New methods allow for more direct assessment of protein synthesis and secretion rates.
    • Advancements provide a more comprehensive understanding of protein regulation.

    Conclusions:

    • Future research will leverage cDNA clones for specific enterocyte proteins to simplify structural analysis.
    • Intestinal cell lines will be crucial for simplifying measurements of synthetic rates and protein processing.