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Updated: Jul 9, 2025
Laser Microdissection for Species-Agnostic Single-Tissue Applications
Published on: March 31, 2022
Protocol for nuclear dissociation of the adult C. elegans for single-nucleus RNA sequencing and its application for
Max T Levenson1, Rio Barrere-Cain2, Lisa Truong3
1Molecular Toxicology Inter-Departmental Program, UCLA, Los Angeles, CA 90095, USA.
Abstract:
Caenorhabditis elegans is a valuable model to study organ, tissue, and cell-type responses to external cues. However, the nematode comprises multiple syncytial tissues with spatial coordinates corresponding to distinct nuclear transcriptomes. Here, we present a single-nucleus RNA sequencing (snRNA-seq) protocol that aims to overcome difficulties encountered with single-cell RNA sequencing in C. elegans. We describe steps for isolating C. elegans nuclei for downstream applications including snRNA-seq applied to the context of alcohol exposure. For complete details on the use and execution of this protocol, please refer to Truong et al. (2023).1.
