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Updated: Jul 9, 2025

ATAC-Seq Library Preparation of Murine Bone Marrow-Derived Neutrophils
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Understanding the Relationship Between Neutrophil Function and Demographic Variables.

Elise Hickman1, Meghan E Rebuli1, Carole Robinette1

  • 1The University of North Carolina at Chapel Hill.

Research Square
|December 4, 2023
PubMed
Summary

Neutrophil function in respiratory health varies by sex. Males show higher phagocytic capacity than females, highlighting sex as a key factor in immune defense research.

Keywords:
Neutrophilbioenergeticsphagocytosissex differences

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Area of Science:

  • Immunology
  • Cellular Biology
  • Respiratory Medicine

Background:

  • Neutrophils are critical for combating respiratory pathogens, and their dysfunction is implicated in airway diseases.
  • Understanding demographic influences on neutrophil function is essential for advancing respiratory host defense strategies.

Approach:

  • Investigated associations between demographic factors (age, BMI, sex) and neutrophil functional phenotypes (oxidative burst, bioenergetics).
  • Measured PMA-stimulated oxidative burst and phagocytosis of human neutrophils using Seahorse XF and pHrodo red S. aureus assays.
  • Analyzed demographic associations with cellular functions, including phagocytic capacity and bioenergetic parameters.

Key Points:

  • No significant associations were found between neutrophil oxidative burst, bioenergetics, or phagocytosis and BMI or age.
  • A notable sexual dimorphism in neutrophil phagocytosis was observed, with males demonstrating significantly higher capacity than females.
  • Phagocytic capacity and bioenergetic parameters correlated in males, but not in females, suggesting sex-specific activation pathways.

Conclusions:

  • The study reveals potential sex-based variations in neutrophil activation pathways.
  • Emphasizes the critical importance of incorporating sex as a biological variable in respiratory host defense research.
  • Suggests further investigation into sex-specific immune responses to respiratory pathogens.