Related Experiment Video

Updated: Jul 9, 2025

Measurement of Force-Sensitive Protein Dynamics in Living Cells Using a Combination of Fluorescent Techniques
08:28

Measurement of Force-Sensitive Protein Dynamics in Living Cells Using a Combination of Fluorescent Techniques

Published on: November 2, 2018

8.3K

Molecular simulation approaches to probing the effects of mechanical forces in the actin cytoskeleton

Fatemah Mukadum, Willmor J Pena Ccoa, Glen M Hocky

    Arxiv
    |December 4, 2023
    PubMed

    More Related Videos

    Probing Myosin Ensemble Mechanics in Actin Filament Bundles Using Optical Tweezers06:53

    Probing Myosin Ensemble Mechanics in Actin Filament Bundles Using Optical Tweezers

    Published on: May 4, 2022

    2.3K
    Reconstituting and Characterizing Actin-Microtubule Composites with Tunable Motor-Driven Dynamics and Mechanics09:10

    Reconstituting and Characterizing Actin-Microtubule Composites with Tunable Motor-Driven Dynamics and Mechanics

    Published on: August 25, 2022

    3.3K

    Related Concept Videos

    Studying the Cytoskeleton01:17

    Studying the Cytoskeleton

    The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...
    6.3K
    Mechanical Protein Functions01:58

    Mechanical Protein Functions

    Proteins perform many mechanical functions in a cell. These proteins can be classified into two general categories- proteins that generate mechanical forces and proteins that are subjected to mechanical forces. Proteins providing mechanical support to the structure of the cell, such as keratin, are subjected to mechanical force, whereas proteins involved in cell movement and transport of molecules across cell membranes, such as an ion pump, are examples of generating mechanical force. 
    4.9K
    Generation of Straight or Branched Actin Filaments01:14

    Generation of Straight or Branched Actin Filaments

    The straight or branched structure formation of actin filaments is controlled by nucleating proteins such as the formins and Arp2/3 complex. Formin-mediated assembly results in straight filaments, whereas Arp2/3 protein complex-mediated assembly results in branched actin filaments.
    Arp2/3 Complex
    Arp2/3 complex is a seven-subunit complex consisting of two proteins similar to actin- Arp2 and Arp3, and five other subunits that help keep Arp2 and Arp3 inactive. When required, the complex is...
    2.9K
    Actin Filament Depolymerization01:19

    Actin Filament Depolymerization

    Actin filaments (F-actin) are composed of actin subunits. The dissociation of actin monomers can occur from either end of F-actin. The rate of dissociation is faster from the minus-end or the pointed end, where the actin subunits exist with a bound ADP, together known as ADP-actin. The depolymerization of F-actin is aided by proteins, including the actin-depolymerizing factor (ADF) and cofilin family of proteins, gelsolin, and glia maturation factor (GMF).
    In F-actin, the ADF/cofilin proteins...
    3.1K
    Actin Polymerization and Cell Motility01:13

    Actin Polymerization and Cell Motility

    Actin is a family of globular proteins that are highly abundant in eukaryotic cells. It makes up approximately 1-5% of total cell protein concentration. Actin monomers polymerize to form a complex network of polarized filaments, the actin cytoskeleton, that plays a crucial role in many cellular processes, including cell motility, division, endocytosis, and metastasis of cancer cells.
    Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
    5.3K
    Cell-matrix's Response to Mechanical Forces01:13

    Cell-matrix's Response to Mechanical Forces

    In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
    Anchoring junctions mechanically attach a cell to the...
    2.6K