Enantioselective Switch and Potential Applications in Biocatalysis
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Updated: Jul 9, 2025

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
Published on: August 8, 2016
Lucia Robustini1, Francesca Paradisi2
1Dept. Chemistry, Biochemistry and Pharmaceutical Sciences. University of Bern, Freiestrasse 3, CH-3012 Bern. lucia.robustini@unibe.ch.
Abstract:
Enantioselectivity has always been a key feature of enzymatic synthesis. In some cases, when enzymes are not strictly enantioselective, by tuning the reaction conditions it is possible to induce an enantioselective switch. A transaminase from Halomonas elongata (ω-HeWT), while generally S-selective, could be shifted towards generating the R-enantiomer at higher concentrations of amino acceptor or ionic strength, for example. Other enzymes are reported to have a similar behavior, and here we discuss some of them and their potential applications.
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