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Related Experiment Video

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Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis
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Periostin regulates integrin expression in gingival epithelial cells.

Reika Hirata1, Tomoyuki Iwata1, Tsuyoshi Fujita1

  • 1Department of Periodontal Medicine, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima 734-8553, Japan.

Journal of Oral Biosciences
|December 4, 2023
PubMed
Summary

Periostin, secreted by human periodontal ligament (HPL) cells and mesenchymal stem cells (MSCs), regulates integrins in human gingival epithelial cells (HGECs). This finding is crucial for maintaining cell adhesion and preventing periodontitis.

Keywords:
Gingival epithelial cellsHuman periodontal ligament cellsIntegrin betaMesenchymal stem cellsPeriostin

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Area of Science:

  • Cell Biology
  • Periodontology
  • Biochemistry

Background:

  • Human gingival epithelial cells (HGECs) form a barrier via cell-cell junctions, involving integrins.
  • Human periodontal ligament (HPL) cells maintain homeostasis, but are lost in periodontitis.
  • Transplantation of HPL cells or mesenchymal stem cells (MSCs) is a potential regenerative therapy.

Purpose of the Study:

  • To investigate the regulatory mechanisms of HGECs by HPL cells and MSCs.
  • To elucidate the role of periostin in maintaining periodontal tissue homeostasis.
  • To understand how cell-cell interactions influence gingival barrier function.

Main Methods:

  • Cultured human gingival fibroblasts (HGFs), HGECs, HPL cells, and MSCs.
  • Collected conditioned medium and performed periostin mRNA silencing.
  • Analyzed integrin and periostin expression via real-time PCR, flow cytometry, and western blotting.

Main Results:

  • Conditioned medium from MSCs and HPL cells increased integrin expression in HGECs.
  • Periostin protein in conditioned medium regulated HGEC integrin expression.
  • Silencing periostin eliminated this effect, indicating periostin's critical role.

Conclusions:

  • Periostin secreted by HPL cells and MSCs regulates integrins in HGECs.
  • Periostin is vital for gingival cell adhesion and defense against bacterial invasion.
  • Regulating periostin-secreting cells is key for periodontitis prevention.