Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

ATP and Macromolecule Synthesis01:28

ATP and Macromolecule Synthesis

5.6K
Biological macromolecules are organic compounds, predominantly composed of carbon atoms. The carbon atoms are covalently bonded with hydrogen, oxygen, nitrogen, and other minor elements. There are four major biological macromolecule classes: carbohydrates, lipids, proteins, and nucleic acids.
Most macromolecules are composed of single subunits, or building blocks, called monomers. The monomers combine with each other using covalent bonds to form larger molecules known as polymers.
Conversion of...
5.6K
Synthetic Biology02:55

Synthetic Biology

4.8K
Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
4.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mapping mRNA Localization and Internal Structure in Lipid Nanoparticles through Solid-State Dynamic Nuclear Polarization NMR and Proton Spin-Diffusion Modeling.

Small methods·2026
Same author

Rapid and Efficient Antibody-Drug Conjugate Design Using Mechanistic Bottom-Up Modeling from In Vitro to Human.

Bioconjugate chemistry·2026
Same author

Hydrogels Incorporating Donor-Acceptor Stenhouse Adducts as a Platform for Photoinduced, On-Off Switchable Release of Small Molecule Cargos.

Macromolecular rapid communications·2026
Same author

Peptide-reinforced, photocrosslinkable PEG-based hydrogels.

RSC applied polymers·2026
Same author

Targeting highly attenuated IL-18 to PD-1 for enhanced anti-tumor activity.

Frontiers in immunology·2026
Same author

A masking clamp for conditional activation of therapeutic antibodies.

Frontiers in immunology·2025

Related Experiment Video

Updated: Jul 9, 2025

Synthesis of Monodisperse Cylindrical Nanoparticles via Crystallization-driven Self-assembly of Biodegradable Block Copolymers
11:42

Synthesis of Monodisperse Cylindrical Nanoparticles via Crystallization-driven Self-assembly of Biodegradable Block Copolymers

Published on: June 20, 2019

7.8K

Artificial cell synthesis using biocatalytic polymerization-induced self-assembly.

Andrea Belluati1,2, Sètuhn Jimaja3, Robert J Chadwick4

  • 1Department of Pure and Applied Chemistry, University of Strathclyde, Thomas Graham Building, Glasgow, UK. andrea.belluati@tu-darmstadt.de.

Nature Chemistry
|December 4, 2023
PubMed
Summary
This summary is machine-generated.

Researchers created polymer-based artificial cells using enzymatic synthesis. These artificial cells can express proteins and mimic bacterial functions, opening new avenues in molecular systems engineering and synthetic biology.

More Related Videos

Preparing Protein Producing Synthetic Cells using Cell Free Bacterial Extracts, Liposomes and Emulsion Transfer
09:37

Preparing Protein Producing Synthetic Cells using Cell Free Bacterial Extracts, Liposomes and Emulsion Transfer

Published on: April 27, 2020

11.1K
Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly
09:34

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Published on: February 6, 2020

7.3K

Related Experiment Videos

Last Updated: Jul 9, 2025

Synthesis of Monodisperse Cylindrical Nanoparticles via Crystallization-driven Self-assembly of Biodegradable Block Copolymers
11:42

Synthesis of Monodisperse Cylindrical Nanoparticles via Crystallization-driven Self-assembly of Biodegradable Block Copolymers

Published on: June 20, 2019

7.8K
Preparing Protein Producing Synthetic Cells using Cell Free Bacterial Extracts, Liposomes and Emulsion Transfer
09:37

Preparing Protein Producing Synthetic Cells using Cell Free Bacterial Extracts, Liposomes and Emulsion Transfer

Published on: April 27, 2020

11.1K
Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly
09:34

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Published on: February 6, 2020

7.3K

Area of Science:

  • Biomimetic materials science
  • Synthetic biology
  • Polymer chemistry

Background:

  • Artificial cells are engineered microstructures designed to replicate natural cell functions.
  • They serve as foundational units for molecular systems engineering and hosting synthetic biology pathways.
  • Current methods often lack the ability to integrate complex cellular functions like protein expression.

Purpose of the Study:

  • To develop novel polymer-based artificial cells capable of protein expression.
  • To utilize enzymatic synthesis for creating these advanced artificial cells.
  • To demonstrate the potential of these artificial cells as microreactors and in mimicking bacterial functions.

Main Methods:

  • Biocatalytic atom transfer radical polymerization-induced self-assembly was employed.
  • Myoglobin was used to synthesize amphiphilic block co-polymers.
  • These polymers self-assembled into various structures, including giant unilamellar vesicles (GUVs), encapsulating diverse cargo.

Main Results:

  • Synthesized polymer-based GUVs capable of encapsulating enzymes, nanoparticles, plasmids, and cell lysate.
  • Demonstrated artificial cell functionality as microreactors for enzymatic reactions and biomineralization.
  • Achieved de novo protein expression (fluorescent protein, actin) within the GUVs upon provision of amino acids.

Conclusions:

  • Enzymatically synthesized GUVs can serve as self-contained microreactors with protein expression capabilities.
  • These artificial cells effectively mimic bacterial structures and functions, including genetic information processing.
  • The study presents a significant advancement in creating sophisticated biomimetic systems for synthetic biology applications.