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3D-Printed Collagen-Nanocellulose Hybrid Bioscaffolds with Tailored Properties for Tissue Engineering Applications
Andreja Dobaj Štiglic1,2, Florian Lackner3, Chandran Nagaraj4
1Faculty of Mechanical Engineering, Laboratory for Characterization and Processing of Polymers, University of Maribor, Smetanova ulica 17, 2000 Maribor, Slovenia.
ACS Applied Bio Materials
|December 5, 2023
Summary
Researchers developed novel hybrid collagen (Coll) bioscaffolds using nanofibrillated cellulose, carboxymethylcellulose, and citric acid for tissue engineering. These biocompatible scaffolds offer enhanced stability and structural support for cell growth.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Hybrid collagen (Coll) bioscaffolds are promising for tissue engineering (TE) and regenerative medicine.
- Combining Coll with other biomaterials enhances mechanical strength and mimics the natural tissue microenvironment.
Purpose of the Study:
- Fabricate chemically cross-linked hybrid bioscaffolds using Coll, nanofibrillated cellulose (NFC), carboxymethylcellulose (CMC), and citric acid (CA).
- Investigate the impact of Coll concentration on scaffold properties for TE applications.
Main Methods:
- 3D printing of a Coll-NFC-CMC-CA ink.
- Freeze-drying, dehydrothermal treatment, and neutralization to form cross-linked bioscaffolds.
- Characterization of morphology, porosity, chemical composition, thermal behavior, swelling, degradation, and mechanical properties.
Main Results:
- Successful fabrication of chemically cross-linked hybrid bioscaffolds via ester bond formation using citric acid.
- Scaffold properties (morphology, mechanical strength, stability) were tunable based on Coll concentration.
- Biocompatibility confirmed through non-cytotoxicity assays with MG-63 human bone osteosarcoma cells.
Conclusions:
- The developed hybrid Coll bioscaffolds exhibit excellent biocompatibility, stability, and structural support.
- These novel bioscaffolds are valuable tools for tissue engineering and regenerative medicine.
- The tunable properties make them adaptable for various TE applications.
