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Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform
Published on: November 10, 2016
In Situ Stability Test for mRNA Vaccines Based on Deep-UV Resonance Raman Spectroscopy
Lamyaa M Almehmadi1,2, Sergei V Reverdatto1,2, Vladimir V Ermolenkov2
1Department of Chemistry, University at Albany, SUNY, 1400 Washington Avenue, Albany, New York 12222, United States.
Abstract:
Deep-UV resonance Raman spectroscopy has been shown to offer great potential for probing the in situ stability of mRNA vaccines. In this study, a vaccine model was subjected to controlled degradation using RNase A or through aging at room temperature. The degradation of mRNA was confirmed by using a cell transfection test and by gel electrophoresis. Under both settings, DUVRR spectroscopy successfully revealed the mRNA degradation signs of the vaccine model.
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