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Sample transformation in online separations: how chemical conversion advances analytical technology.

Annika A M van der Zon1,2, Joshka Verduin2,3, Rick S van den Hurk1,2

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Chemical transformation enhances analytical methods for complex mixtures by reducing complexity and improving detection. This approach provides new insights into sample properties like degradation and heterogeneity.

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Area of Science:

  • Analytical Chemistry
  • Chemical Analysis
  • Sample Preparation

Background:

  • Modern analytical technologies reveal complex mixtures.
  • Understanding these sophisticated mixtures requires advanced techniques.
  • Chemical transformation offers a powerful approach to gain deeper insights into sample properties.

Purpose of the Study:

  • To explore the application of sample transformation in analytical chemistry.
  • To demonstrate how chemical transformation empowers analytical methods for complex samples.
  • To highlight the state-of-the-art and future prospects of sample transformation techniques.

Main Methods:

  • Chemical transformation mechanisms including molecular-weight reduction, controlled degradation, and derivatization.
  • Exploration of both offline and online transformation methods.
  • Application across diverse fields such as therapeutics, nanoparticles, polymers, and small molecules.

Main Results:

  • Sample transformation facilitates significantly faster reaction times.
  • It effectively reduces sample complexity, simplifying analysis.
  • New analytical dimensions, such as functional groups, are unlocked.
  • Correlations between multiple sample dimensions are established.
  • Overall detectability of analytes is improved.

Conclusions:

  • Sample transformation is a key strategy for enhancing the analysis of complex mixtures.
  • It provides previously inaccessible information about sample properties.
  • This approach holds significant promise for future analytical advancements.